CD93 抗体
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- 抗原 See all CD93 抗体
- CD93
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD93 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- Monoclonal antibody X-2 recognizes a 110 kDa O-sialoglycoprotein on granulocytes, monocytes and endothelial cells. Upon differentiation of cultivated monocytes into macrophages, CDw93 ceases to be expressed on day 5-6, which makes it a very useful marker to differentiate those two cell populations. Antigen distribution on isolated cells: Positive with all granulocytes, with staining increasing transiently upon maturation. Antigen expression is increased by TNFα, GM-CSF, (fMLP) treatment. No effect on superoxide production by binding to neutrophils has been noted. PMA stimulation causes down regulation of the antigen. Positive with monocytes following fMLP, TNF, GM-CSF treatment. Antigen expression decreases on cultured monocytes. Positive on AML cases with monocytic component. Positive on cell lines HL-60, Namalva, K562, THP-1, U937, negative on HMC-1 (human mast cell line). Antigen distribution on tissue sections: Monoclonal antibody X-2 stains epitheloid cells and Rosai-Dorfman cells and most endothelial cells in the synovial membrane of RA patients.
- 纯化方法
- Affinity chromatography.
- 免疫原
- Human monocytes
- 克隆位点
- X-2
- 亚型
- IgG1
- Top Product
- Discover our top product CD93 Primary Antibody
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anti-CD93 (CD93) (AA 474-535) antibody
CD93 适用: 人 WB, IHC, FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 1A10E10 unconjugated
anti-CD93 (CD93) (AA 474-535) antibodyCD93 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 2F7D11 unconjugated
anti-CD93 (CD93) (AA 82-369) antibodyCD93 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD93 (CD93) antibodyCD93 适用: 人 FACS, IP, IHC (fro) 宿主: 小鼠 Monoclonal mNI-11 unconjugated
anti-CD93 (CD93) (Internal Region) antibodyCD93 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD93 (CD93) (AA 227-493) antibodyCD93 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD93 (CD93) (AA 33-140) antibodyCD93 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1A4 unconjugated
anti-CD93 (CD93) (C-Term) antibodyCD93 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD93 (CD93) antibodyCD93 适用: 人 WB, IHC, FACS, IP, ICC 宿主: 小鼠 Monoclonal VIMD2 unconjugated
anti-CD93 (CD93) antibody (FITC)CD93 适用: 人 FACS 宿主: 小鼠 Monoclonal VIMD2 FITC
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- 应用备注
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Tested for immunohistochemistry (IHC, Frozen sections: 2 μg/mL (1: 100), Does not react onroutinely processed paraffin sections. Suggested positive control: Human tonsil. Has been described to work in FACS.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain
- 限制
- 仅限研究用
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- 溶解方式
- Restore with 0.5 mL distilled water.
- 浓度
- 0.2 mg/mL IgG
- 缓冲液
- PBS, pH 7.2 with 10 mg/mL bovine serum albumin (BSA) as a stabilizer and 0.01 % Thimerosal as a preservative.
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store the antibody at -20 °C. Ship at 2-8 °C.
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- 抗原
- CD93
- 别名
- CD93 / C1QR1 (CD93 产品)
- 别名
- 6030404G09Rik antibody, AA145088 antibody, AA4.1 antibody, AW555904 antibody, C1qr1 antibody, C1qrp antibody, Ly68 antibody, C1QR1 antibody, C1qR(P) antibody, C1qRP antibody, CDw93 antibody, ECSM3 antibody, MXRA4 antibody, dJ737E23.1 antibody, C1qRp antibody, CD93 antigen antibody, CD93 molecule antibody, Cd93 antibody, CD93 antibody
- 背景
- CD93 a cell-surface glycoprotein and type I membrane protein involve in intercellular adhesion and in the clearance of apoptotic cells. The intracellular cytoplasmic tail of this protein has been found to interact with moesin, a protein known to play a role in linking transmembrane proteins to the cytoskeleton and in the remodelling of the cytoskeleton.Synonyms: C1q receptor, C1q/MBL/SPA receptor, CDw93, Complement component 1 q subcomponent receptor 1, Complement component C1q receptor, MBL receptor, MXRA4, Matrix-remodeling-associated protein 4, SPA receptor
- 基因ID
- 22918
- NCBI登录号
- NP_036204
- UniProt
- Q9NPY3
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