CD93 抗体
Quick Overview for CD93 抗体 (ABIN111895)
抗原
See all CD93 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Monoclonal antibody X-2 recognizes a 110 kDa O-sialoglycoprotein on granulocytes, monocytes and endothelial cells. Upon differentiation of cultivated monocytes into macrophages, CDw93 ceases to be expressed on day 5-6, which makes it a very useful marker to differentiate those two cell populations. Antigen distribution on isolated cells: Positive with all granulocytes, with staining increasing transiently upon maturation. Antigen expression is increased by TNFα, GM-CSF, (fMLP) treatment. No effect on superoxide production by binding to neutrophils has been noted. PMA stimulation causes down regulation of the antigen. Positive with monocytes following fMLP, TNF, GM-CSF treatment. Antigen expression decreases on cultured monocytes. Positive on AML cases with monocytic component. Positive on cell lines HL-60, Namalva, K562, THP-1, U937, negative on HMC-1 (human mast cell line). Antigen distribution on tissue sections: Monoclonal antibody X-2 stains epitheloid cells and Rosai-Dorfman cells and most endothelial cells in the synovial membrane of RA patients.
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纯化方法
- Affinity chromatography.
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免疫原
- Human monocytes
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亚型
- IgG1
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anti-CD93 (CD93) (AA 474-535) antibody
CD93 适用: 人 IHC, ICC, FACS, ELISA 宿主: 小鼠 Monoclonal 1A10E10 unconjugated
anti-CD93 (CD93) (AA 474-535) antibodyCD93 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 2F7D11 unconjugated
anti-CD93 (CD93) (AA 82-369) antibodyCD93 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD93 (CD93) antibodyCD93 适用: 人 IP, FACS, IHC (fro) 宿主: 小鼠 Monoclonal mNI-11 unconjugated
anti-CD93 (CD93) (AA 227-493) antibodyCD93 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD93 (CD93) (C-Term) antibodyCD93 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD93 (CD93) (AA 33-140) antibodyCD93 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1A4 unconjugated
anti-CD93 (CD93) antibodyCD93 适用: 人 WB, IHC, IP, ICC, FACS 宿主: 小鼠 Monoclonal VIMD2 unconjugated
anti-CD93 (CD93) antibody (FITC)CD93 适用: 人 FACS 宿主: 小鼠 Monoclonal VIMD2 FITC
anti-CD93 (CD93) antibody (PE)CD93 适用: 人 FACS 宿主: 小鼠 Monoclonal VIMD2 PE
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应用备注
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Tested for immunohistochemistry (IHC, Frozen sections: 2 μg/mL (1: 100), Does not react onroutinely processed paraffin sections. Suggested positive control: Human tonsil. Has been described to work in FACS.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain
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限制
- 仅限研究用
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溶解方式
- Restore with 0.5 mL distilled water.
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浓度
- 0.2 mg/mL IgG
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缓冲液
- PBS, pH 7.2 with 10 mg/mL bovine serum albumin (BSA) as a stabilizer and 0.01 % Thimerosal as a preservative.
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储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody at -20 °C. Ship at 2-8 °C.
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- CD93
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别名
- CD93 / C1QR1
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背景
- CD93 a cell-surface glycoprotein and type I membrane protein involve in intercellular adhesion and in the clearance of apoptotic cells. The intracellular cytoplasmic tail of this protein has been found to interact with moesin, a protein known to play a role in linking transmembrane proteins to the cytoskeleton and in the remodelling of the cytoskeleton.Synonyms: C1q receptor, C1q/MBL/SPA receptor, CDw93, Complement component 1 q subcomponent receptor 1, Complement component C1q receptor, MBL receptor, MXRA4, Matrix-remodeling-associated protein 4, SPA receptor
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基因ID
- 22918
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NCBI登录号
- NP_036204
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UniProt
- Q9NPY3
抗原
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