S100A9 抗体 (C-Term) (Biotin)
(2 references)-
- 抗原 See all S100A9 抗体
- S100A9 (S100 Calcium Binding Protein A9 (S100A9))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This S100A9 antibody is conjugated to Biotin
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- 特异性
- Monoclonal antibody S32.2 identifies the Ca2+-binding 14 kDa subunit of the inflammatory L-1 protein complex, also called S100A9 or Calgranulin B. It is useful for the characterization of circulating granulocytes or inflammatory infiltrates of the myelo-monocytic lineage which express MRP14 differently depending on the inflammatory status of the disease. S32.2 detects a very distinct epitope on the C-terminal end of MRP14 (different from the S 36.48 epitope). The epitope is stable to 0.1 % glutaraldehyde or formalin fixation, and paraffin embedding. Antigen Distribution Isolated cells: The antigen is found in granulocytes and monocytes. It is absent from all other blood cells. In cultured monocytes, maximum MRP-14 is expressed after 3-4 days. Myeloid leukaemia cells have been found to be positive as well. Tissue Sections: MRP-14 is only found in a distinct subpopulation of inflammatory perivascular infiltrates of the myelo-monocytic lineage. Reports from the literature indicate that macrophages synthesise MRP-14 increasingly during the early stages of inflammation. A high MRP-14 (and low MRP-8) expression by macrophages was also reported in granulomatous diseases such as tuberculosis and sarcoidosis. In non-granulomatous chronic inflammatory diseases such as chronic rheumatoid arthritis MRP-8 and MRP-14 positive cells consist of different subpopulations. During early inflammation endothelial cells are also positive with MRP-8/14 determined by antibody 27E10 (Cat.-No BM4025).Antigen Distribution
- 交叉反应 （详细）
- Species reactivity (tested):Human: MRP14, Granulocytes, stimulated Monocytes and Macrophages. Does not react with Bovine and Swine spleen.
- 纯化方法
- Protein A Chromatography
- 免疫原
- Cultured Human Monocytes. Remarks: The antigen is MRP14, the epitope is suspected in the carboxyterminal portion of the peptide.
- 克隆位点
- S32-2
- 亚型
- IgG1
- Top Product
- Discover our top product S100A9 Primary Antibody
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anti-S100 Calcium Binding Protein A9 (S100A9) antibody
S100A9 适用: 大鼠, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A9 (S100A9) (Internal Region) antibodyVerified S100A9 适用: 人 WB, IHC, ELISA, FACS, IF 宿主: 山羊 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A9 (S100A9) antibodyS100A9 适用: 人, 大鼠, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A9 (S100A9) (AA 1-114) antibodyS100A9 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A9 (S100A9) (AA 51-114) antibodyS100A9 适用: 人 ELISA, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A9 (S100A9) antibodyS100A9 适用: 人, 大鼠 IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal S100A9-1075 unconjugated
anti-S100 Calcium Binding Protein A9 (S100A9) (AA 1-114) antibodyS100A9 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1C10 unconjugated
anti-S100 Calcium Binding Protein A9 (S100A9) (AA 2-113) antibodyS100A9 适用: 大鼠, 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A9 (S100A9) (AA 2-114) antibodyS100A9 适用: 人 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A9 (S100A9) (AA 1-114) antibodyS100A9 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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ELISA. Flow Cytometry. Immunohistochemistry (IHC) on: Frozen sections: 0.25 μg/mL (1/800)Paraffin sections: 2 μg/mL (1/100), no pretreatment for antigen retrieval necessary. Suggested positive control: Human tonsil. Has been described to work Dot blots.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore in 0.5 mL distilled water to make stock solution.
- 浓度
- 0.2 mg/mL
- 缓冲液
- PBS, pH 7.2 with 5 mg/mL BSA as a stabilizer and kathon as a preservative
- 储存液
- Kathon CG
- 注意事项
- This product contains Kathon CG a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 注意事项
- This product is photosensitive and should be protected from light
- 储存条件
- 4 °C
- 储存方法
- Store the antibody undiluted undiluted at 2-8 °C. DO NOT FREEZE!
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: "Tandem affinity depletion: a combination of affinity fractionation and immunoaffinity depletion allows the detection of low-abundance components in the complex proteomes of body fluids." in: Journal of proteome research, Vol. 9, Issue 12, pp. 6126-34, (2010) (PubMed).
: "A novel regulator of telomerase. S100A8 mediates differentiation-dependent and calcium-induced inhibition of telomerase activity in the human epidermal keratinocyte line HaCaT." in: The Journal of biological chemistry, Vol. 282, Issue 9, pp. 6126-35, (2007) (PubMed).
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: "Tandem affinity depletion: a combination of affinity fractionation and immunoaffinity depletion allows the detection of low-abundance components in the complex proteomes of body fluids." in: Journal of proteome research, Vol. 9, Issue 12, pp. 6126-34, (2010) (PubMed).
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- 抗原
- S100A9 (S100 Calcium Binding Protein A9 (S100A9))
- 别名
- S100A9 / Calgranulin-B / MRP14 (S100A9 产品)
- 别名
- 60B8AG antibody, CAGB antibody, CFAG antibody, CGLB antibody, L1AG antibody, LIAG antibody, MAC387 antibody, MIF antibody, MRP14 antibody, NIF antibody, P14 antibody, Mrp14 antibody, S100A9 antibody, MRP-14 antibody, p14 antibody, 60B8Ag antibody, AW546964 antibody, BEE22 antibody, Cagb antibody, GAGB antibody, L1Ag antibody, S100 calcium binding protein A9 antibody, S100 calcium binding protein A9 (calgranulin B) antibody, S100A9 antibody, S100a9 antibody
- 背景
- S100A9 is a member of the S100 family of proteins. S100A9, together with S100A8 forms a heterodimeric protein complex, Calprotectin, which is a major calcium- and zinc-binding protein in the cytosol of neutrophils, monocytes, and keratinocytes. Complexes of S100A8 and S100A9 are the physiologically relevant forms of these proteins. S100A9 may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis. Its expression and potential cytokine-like function in inflammation and in cancer suggest that S100A8/A9 may play a key role in inflammation-associated cancer.Synonyms: CAGB, Calprotectin L1H subunit, Leukocyte L1 complex heavy chain, MRP-14, Migration inhibitory factor-related protein 14, S100 calcium-binding protein A9, S100-A9
- 基因ID
- 9606
- UniProt
- P06702
- 途径
- Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins
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