Fibroblasts (Reticular) 抗体
适用: 小鼠, 人, Pig
IF, IHC (fro)
宿主: 大鼠
Monoclonal
ER-TR7
unconjugated
1 image
(21 references)-
- 抗原
- Fibroblasts (Reticular)
- 适用
- 小鼠, 人, Pig
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- Reacts with Mouse reticular fibroblasts, reticular fibres and Human tonsil. ER-TR7 detects an antigen present in and produced by reticular fibroblasts. The recognized antigen is most likely distinct from Laminin, Fibronectin, Collagen types I-IV, Heparan Sulfate Proteoglycan, Entactin, and Nidogen. The antibody is useful to stain the microanatomy of various organs, in particular the connective tissue framework in lymphoid organs. The antibody also stains subendothelial deposits in the plaque area of atherosclerotic plaques. Antigen Distribution Isolated Cells: The antigen is found in the cytoplasm of fibroblast cell lines. Tissue Sections: The antigen is expressed in connective tissues which form a supporting network between parenchymal cells of all organs tested so far (see table below). Thus, the supportive mesenchymal structures of larger vessels can be studied. In spleen, a very clear delineation of red and white pulp is obtained. Capsule, sinuses, follicules, paracortex and medullary cords are also clearly delineated in lymph nodes.Antigen Distribution
- 交叉反应 （详细）
- Species reactivity (tested):Mouse (Reticular Fibroblasts, Reticular Fibres), Pig and Human.
- 纯化方法
- Affinity Chromatography
- 免疫原
- Murine thymic reticulum. Epitope: The antigen has not been fully characterized. The epitope may be part of reticulin.
- 克隆位点
- ER-TR7
- 亚型
- IgG2a
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anti-Fibroblasts (Reticular) antibody
适用: 小鼠 IF, FACS, IHC (p) 宿主: 大鼠 Monoclonal ER-TR7 unconjugated
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- 应用备注
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Immunofluorescence. Immunohistochemistry on Frozen Sections: 1 μg/mL (1/400) freshly prepared for Mousetissue and 4 μg/mL (1/100) on Human/Porcine tissue. Recommended Positive Control: Mouse spleen. Does not react on routinely processed Paraffin Sections. Has been described to work in FACS.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- Protocol with frozen, ice-cold acetone-fixed sections: (The whole procedure is performed at room temperature)1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti rat IgG (H+L)minimal-cross reaction to mouse) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayers hemalum
- 限制
- 仅限研究用
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- 溶解方式
- Restore with 0.5 mL distilled water.
- 浓度
- 0.4 mg/mL
- 缓冲液
- Stock solution contains PBS, pH 7.2 with 5 mg/mL BSA as a stabilizer and 0.09 % Sodium Azide as preservative
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
- 储存条件
- 4 °C
- 储存方法
- Store the antibody undiluted at 4-8 °C for one month or at -20 °C for longer.
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: "B-cell follicle development remodels the conduit system and allows soluble antigen delivery to follicular dendritic cells." in: Blood, Vol. 114, Issue 24, pp. 4989-97, (2009) (PubMed).
: "Fibroblastic reticular cells guide T lymphocyte entry into and migration within the splenic T cell zone." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 181, Issue 6, pp. 3947-54, (2008) (PubMed).
: "Pulmonary expression of CXC chemokine ligand 13, CC chemokine ligand 19, and CC chemokine ligand 21 is essential for local immunity to influenza." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 25, pp. 10577-82, (2007) (PubMed).
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: "Distinct mouse bone marrow macrophage precursors identified by differential expression of ER-MP12 and ER-MP20 antigens." in: European journal of immunology, Vol. 24, Issue 10, pp. 2279-84, (1994) (PubMed).
: "Expression of the DBA/2J Ren-2 gene in the adrenal gland of transgenic mice." in: The EMBO journal, Vol. 8, Issue 13, pp. 4065-72, (1990) (PubMed).
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: "Laminin regulates PDGFRβ(+) cell stemness and muscle development." in: Nature communications, Vol. 7, pp. 11415, (2016) (PubMed).
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- 抗原
- Fibroblasts (Reticular)
- 背景
- Connective tissue consists of a ground of glycosaminoglycans, proteoglycans and glycoproteins through which various fibres run. These fibres can be collagenous, elastic or reticular. Reticular fibres are composed from the family of collagen proteins and give tensile strength. These fibres are made by reticular fibroblasts.Synonyms: Fibroblast Marker, Fibroblasten
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