LY75/DEC-205 抗体
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- 抗原 See all LY75/DEC-205 (LY75) 抗体
- LY75/DEC-205 (LY75) (Lymphocyte Antigen 75 (LY75))
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This LY75/DEC-205 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- Reacts with nonlymphoid dendritic cells: interdigitating cells (IDC), veiled cells and Langerhans cells, thymic epithelial cells. Monoclonal antibody NLDC-145 identifies Ia positive interdigitating cells, veiled cells and Langerhans cells of the skin and their in vitro counterparts. The antigen is expressed at high levels by dendritic cells and thymic epithelial cells. The antigen detected by NLDC-145 is an integral membrane glycoprotein with an apparent mass of 205 kDa, also known as DEC-205.
- 纯化方法
- Affinity Chromatography.
- 免疫原
- Mouse lymph node tissue.
- 克隆位点
- NLDC145
- 亚型
- IgG2a
- Top Product
- Discover our top product LY75 Primary Antibody
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anti-Lymphocyte Antigen 75 (LY75) antibody (PE)
LY75 适用: 人 FACS 宿主: 小鼠 Monoclonal HD30 PE
anti-Lymphocyte Antigen 75 (LY75) antibodyLY75 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 4B10E11 unconjugated
anti-Lymphocyte Antigen 75 (LY75) (AA 440-680) antibodyLY75 适用: 人 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte Antigen 75 (LY75) antibodyLY75 适用: 人 WB, FACS, IHC (fro) 宿主: 小鼠 Monoclonal HD30 unconjugated
anti-Lymphocyte Antigen 75 (LY75) (AA 1520-1666) antibodyLY75 适用: 人 ELISA, WB, FACS, IHC, ICC, Neut 宿主: 小鼠 Monoclonal 2F12E8 unconjugated
anti-Lymphocyte Antigen 75 (LY75) (C-Term) antibodyLY75 适用: 人 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte Antigen 75 (LY75) (Internal Region) antibodyLY75 适用: 人, 小鼠 ELISA, WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte Antigen 75 (LY75) antibody (APC)LY75 适用: 人 FACS 宿主: 小鼠 Monoclonal HD30 APC
anti-Lymphocyte Antigen 75 (LY75) (AA 646-675) antibodyLY75 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB23558 unconjugated
anti-Lymphocyte Antigen 75 (LY75) (AA 37-135) antibodyLY75 适用: 人 ELISA 宿主: 小鼠 Monoclonal 3G4 unconjugated
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- 应用备注
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Immunohistochemistry on Frozen Sections: 0.5 μg/mL (1/400). Suggested Positive Control: Mouse spleen. Does not react on routinely processed paraffin sections. Has been described to work in FACS.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti rat IgG (H+L)minimal-cross reaction to mouse) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum.
- 限制
- 仅限研究用
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- 溶解方式
- Restore by adding 0.5 mL distilled water.
- 浓度
- 0.2 mg/mL
- 缓冲液
- PBS, pH 7.2 containing 5 mg/mL BSA as a stabilizer and 0.02 % Sodium Azide as a preservative.
- 储存液
- Sodium azide
- 注意事项
- Do Not Use Sodium Azide as preservative.
- 注意事项
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
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- 抗原
- LY75/DEC-205 (LY75) (Lymphocyte Antigen 75 (LY75))
- 别名
- CD205 / DEC-205 (LY75 产品)
- 别名
- LY75 antibody, MGC140308 antibody, DEC205 antibody, im:7150926 antibody, si:dkey-78p20.2 antibody, LYT75 antibody, CD205 antibody, DEC-205 antibody, CLEC13B antibody, GP200-MR6 antibody, LY-75 antibody, RGD1565962 antibody, Ly-75 antibody, lymphocyte antigen 75 antibody, CD205 protein antibody, CD302 antigen antibody, LY75 antibody, Cyan7822_2917 antibody, LY5 antibody, ly75 antibody, cd205 antibody, LOC100380704 antibody, LOC100433861 antibody, LOC100483541 antibody, Ly75 antibody
- 背景
- Mouse CD205, also known as DEC-205 (dendritic and epithelial cells, 205 kDa). CD205 is an integral membrane glycoprotein involved in antigen uptake, trafficking and presentation that improves the induction of antigen-specific T cell immunity. CD205 is highly expressed by CD8+ dendritic cells (DCs) and also expressed at different levels by bone marrow Gr1+ cells, Langerhans cells, (BMDC) bone marrow derived DCs and thymic epithelial cells. DEC-205 is apparently a receptor involved in antigen-processing by dendritic cells.Synonyms: C-type lectin domain family 13 member B, CLEC13B, DEC205, LY75, Lymphocyte antigen 75, gp200-MR6
- 基因ID
- 17076
- NCBI登录号
- NP_038853
- UniProt
- Q60767
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