Granulocytes 抗体 (Biotin)
适用: 大鼠
FACS, IHC (p), IHC (fro)
宿主: 小鼠
Monoclonal
RK-4
Biotin
1 image
-
- 抗原
- Granulocytes
-
适用
- 大鼠
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- Biotin
-
应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 纯化方法
- Affinity Chromatography
- 免疫原
- Peritoneal cells.
- 克隆位点
- RK-4
- 亚型
- IgG1
-
anti-Granulocytes antibody
适用: 人, Macaque FACS, IHC (p), IF 宿主: 小鼠 Monoclonal SPM250 unconjugated
-
- 应用备注
-
Flow cytometry: 1/50-1/100. Immunohistochemistry on frozen sections: 0.75 μg/mL (1/400),does not react on routinely processed paraffin sections. Suggested positive control: Rat spleen, blood smears. Antigen Distribution on Isolated Cells: Only a low percentage of cells from peripheralblood stains positive. Positive on peritoneal cells 18h after thioglycollate injection (95 % granulocytes). Negative on peritoneal cells 4 days after thioglycollate injection. Negative on isolated lymphocytes, monocytes,erythrocytes and platelets. See Table 1. for details.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
-
- 溶解方式
- Restore with 0.5 mL distilled water.
- 浓度
- 0.3 mg/mL IgG
- 缓冲液
- Stock Solution contains PBS, pH 7.2 with 0.01 % Thimerosal as preservative and 10 mg/mL BSA as stabilizer
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
-
- 抗原
- Granulocytes
- 背景
- Granulocytes are a type of white blood cell filled with microscopic granules that are little sacs containing enzymes, compounds that digest microorganisms. They are part of the innate immune system and have somewhat nonspecific, broad-based activity. They do not respond exclusively to specific antigens, as do B-cells and T-cells. Neutrophils, eosinophils and basophils are all types of granulocytes. They are named by the staining features of their granules in the laboratory: Neutrophils have "neutral" subtle granules, Eosinophils have prominent granules that stain readily with the acid dye eosin, and Basophils have prominent granules that stain readily basic (non acidic) dyes.Synonyms: Granulocyte cells
-
You are here:
