CD209b Antigen (CD209B) 抗体
(1 reference)Quick Overview for CD209b Antigen (CD209B) 抗体 (ABIN111861)
抗原
See all CD209b Antigen (CD209B) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- ER-TR9 antibody is a very useful marker for the identification of macrophage subpopulations present in the splenic marginal zone, lymph node medulla and other organs. In combination with MOMA-1 (Cat-Nr SM066P), the murine metallophilic macrophage marker, a detailed characterization of murine splenic marginal zone macrophages is obtained. ER-TR9 is also useful when studying phagocytosis of neutral polysaccharides since the antibody selectively inhibits uptake of these glycans by macrophages. The antigen recognized by ER-TR9 has recently been shown to be the Murine analogue of the Human DC-SIGN (Dendritic Cell - Specific ICAM-3 Grabbing Non Integrin), named SIGN-R1. Antigen Distribution Antigen Distribution on Isolated cells: The antigen is found on a subpopulation of phagocytic macrophages isolated from the spleen and showing acid phosphatase and moderate non-specific esterase activity. These phagocytes selectively ingest neutral polysaccharides such as Ficoll. Antigen Distribution on Tissue Sections: Subpopulation of resident macrophages in the splenic marginal zone which are in the proximity of a certain B cell subpopulation (μ+, d-). It is also found on a subpopulation of macrophages localized in the medullary sinuses and trabecular sinuses of lymph nodes. Furthermore, macrophage subpopulations in other organs, such as some connective tissue macrophages in the dermis, may also show ER-TR9 antigen expression. This antibody is specific for a Mouse subpopulation of mature tissue macrophages present in the splenic marginal zone, lymph node medullary and trabecular sinuses.
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交叉反应 （详细）
- Species reactivity (tested):Mouse: Subpopulation of mature tissue macrophages present in the splenic marginal zone, lymph node medullary and trabecular sinuses.
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纯化方法
- Affinity Chromatography
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免疫原
- Thymus cells. Remarks: The antigen is a Glutaraldehyde (0.05%) resistant protein expressed in the cytoplasm and on the cell surface.
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亚型
- IgM
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anti-CD209b Antigen (CD209B) (AA 51-150) antibody
CD209B 适用: 小鼠 WB, IF (cc), IF (p), IHC (fro), IHC (p), ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD209b Antigen (CD209B) antibody (APC)CD209B 适用: 小鼠 FACS 宿主: Armenian Hamster Monoclonal 22D1 APC
anti-CD209b Antigen (CD209B) antibody (PE)CD209B 适用: 小鼠 FACS 宿主: Armenian Hamster Monoclonal 22D1 PE
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应用备注
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Immunohistchemistry on Frozen Sections: 0.5 μg/mL (1/600). Does not react on routinely processed Paraffin sections. Recommended Positive Control: Mouse spleen. Has been described to work in FACS.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 0.5 mL distilled water.
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浓度
- 0.3 mg/mL
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缓冲液
- Stock solution contains PBS, pH 7.2 with 0.1 % Kathon as a preservative
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储存液
- Kathon CG
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注意事项
- This product contains Kathon CG a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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: "Unique requirements for reactivation of virus-specific memory B lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 185, Issue 7, pp. 4011-21, (2010) (PubMed).
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: "Unique requirements for reactivation of virus-specific memory B lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 185, Issue 7, pp. 4011-21, (2010) (PubMed).
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- CD209b Antigen (CD209B)
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别名
- CD209b / DC-SIGNR1
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背景
- Synonyms: CD209 antigen-like protein B, DC-SIGN-related protein 1, OtB7
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基因ID
- 69165
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NCBI登录号
- NP_001032889
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UniProt
- Q8CJ91
抗原
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