SFTPD 抗体 (Lectin Domain)
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Quick Overview for SFTPD 抗体 (Lectin Domain) (ABIN111841)
抗原
See all SFTPD 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- Lectin Domain
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特异性
- Clone VI F11 is useful for staining rat and human surfactant protein D . Antigen Distribution on Tissues and Cells: Alveolar type II cells, alveolar macrophages, two types of Clara cells SP-A+ and SP-A-, and extracellular deposits. Alveolar type III cells stain negative. No reaction on Rat skin, colon, kidney and liver.
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交叉反应 （详细）
- Species reactivity (tested):Rat and Human. Negative with Pig
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纯化方法
- Affinity Chromatography
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免疫原
- Purified Rat Surfactant Protein D (SP-D) from bronchoalveolar lavage. Remarks: The epitope is localized in the lectin domain of SP-D.
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亚型
- IgG1
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anti-Surfactant Protein D (SFTPD) (AA 301-375) antibody
SFTPD 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Surfactant Protein D (SFTPD) (AA 259-374) antibodySFTPD 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Surfactant Protein D (SFTPD) (AA 292-321), (C-Term) antibodySFTPD 适用: 人, 大鼠 WB, IHC, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Surfactant Protein D (SFTPD) (AA 21-375) antibodySFTPD 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Surfactant Protein D (SFTPD) (AA 21-375) antibodySFTPD 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2C10 unconjugated
anti-Surfactant Protein D (SFTPD) antibodySFTPD 适用: Pig WB, IHC (p), IHC (fro), IF 宿主: 小鼠 Monoclonal 1-7 unconjugated
anti-Surfactant Protein D (SFTPD) (AA 21-375) antibodySFTPD 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2A10 unconjugated
anti-Surfactant Protein D (SFTPD) (AA 45-221) antibodySFTPD 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Surfactant Protein D (SFTPD) (AA 50-64) antibodySFTPD 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Surfactant Protein D (SFTPD) (AA 21-375) antibodySFTPD 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
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应用备注
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Immunohistochemistry on Frozen Sections: 0.5 μg/mL (1/800) for Rat Cryosections and10-20 μg/mL (1/20-1/40) for Human Frozen Sections. Immunohistochemistry on Paraffin Sections: 10-20 μg/mL (1/20-1/40) for Rat paraffinsections (microwave pretreatment for antigen retrieval is recommended). Suggested Positive Control: Rat lung. Has been described to work in ELISA and Western blots.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore by adding 0.5 mL distilled water (= 0.4 mg/mL Stock Solution).
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浓度
- 0.4 mg/mL
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缓冲液
- Stock solution contains PBS, pH 7.2 with 0.09 % Sodium Azide as preservative and 5 mg/mL BSA as stabilizer
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C.
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- SFTPD (Surfactant Protein D (SFTPD))
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别名
- SFTPD
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背景
- Surfactant protein D is a Ca2+ -dependent carbohydrate binding protein with a similar structure as other C-type mammalian lectins such as conglutinin and SP-A. The molecular weight is 620 kD under non reducing conditions and 43 kD under reducing conditions. The function of SP-D is not clearly defined. SP-D enhances the production of oxygen radicals in alveolar macrophages and regulates some actions of SP-A, which is the most abundant surfactant protein. SP-D is synthesized and secreted by alveolar epithelial type II cells.Synonyms: COLEC7, Collectin-7, Lung surfactant protein D, PSP-D, PSPD, Pulmonary surfactant-associated protein D, SFTP4, SP-D
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基因ID
- 25350
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NCBI登录号
- NP_037010
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UniProt
- P35248
抗原
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