Sialoadhesin/CD169 抗体
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- 抗原 See all Sialoadhesin/CD169 (SIGLEC1) 抗体
- Sialoadhesin/CD169 (SIGLEC1) (Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1))
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适用
- 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Sialoadhesin/CD169 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- This antibody recognises the CD169 cell surface antigen. CD169 is a 185 kDa molecule expressed by macrophages, predominately confined to lymphoid organs only. Monocytes and granulocytes are negative. No other cell types are positive. The most conspicious property of ED3 is it stains marginal zone macrophages and marginal metallophils in the spleen very strongly. Furthermore, macrophages in (auto-immune) diseased tissues express the ED3 antigen. In healthy tissue no expression occurs. CD169 is a receptor for glycoconjugates containing sialic acid.
- 纯化方法
- Affinity Chromatography on Protein G.
- 免疫原
- Rat Spleen cell homogenate. Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0-Ag 14 mouse myeloma cell line.
- 克隆位点
- ED3
- 亚型
- IgG2a
- Top Product
- Discover our top product SIGLEC1 Primary Antibody
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anti-Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) (AA 851-950) antibody
SIGLEC1 适用: 人, 大鼠 FACS, ELISA, IHC (fro), IF (cc), IF (p), ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) (AA 20-197) antibodySIGLEC1 适用: 人 FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 2A4D1 unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) (AA 572-697) antibodySIGLEC1 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) antibody (APC)SIGLEC1 适用: 人 FACS 宿主: 小鼠 Monoclonal 7-239 APC
anti-Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) antibodySIGLEC1 适用: 大鼠 FACS, IP, IHC (fro), IF 宿主: 小鼠 Monoclonal ED3 unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) antibodySIGLEC1 适用: 人 FACS, WB, IP, IHC (fro) 宿主: 小鼠 Monoclonal 7-239 unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) antibody (PE)SIGLEC1 适用: 人 FACS 宿主: 小鼠 Monoclonal 7-239 PE
anti-Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) (AA 851-950) antibody (AbBy Fluor® 647)SIGLEC1 适用: 人, 大鼠 FACS, IF (cc), IF (p) 宿主: 兔 Polyclonal AbBy Fluor® 647
anti-Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) (Internal Region) antibodySIGLEC1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) (N-Term) antibodySIGLEC1 适用: 人 FACS, WB, IHC, IF, StM 宿主: 小鼠 Monoclonal HSn 7D2 unconjugated
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- 应用备注
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Immunoprecipitation. Flow Cytometry: Use 10 μL of 1/500 diluted CD169 antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen Sections: 1/250.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- Sialoadhesin/CD169 (SIGLEC1) (Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1))
- 别名
- CD169 / SIGLEC1 (SIGLEC1 产品)
- 背景
- Two families of mammalian lectin like adhesion molecules have been shown to bind glycoconjugate ligands in a sialic acid dependent manner: the selectins and the sialoadhesins. The sialoadhesin family has 4 members: CD22, a B cell specific marker, myelin associated glycoprotein (MAG), which is expressed on oligodendrocytes and Schwann cells, CD33, a myeloid differentiation antigen, and sialoadhesin, which is expressed only by a subpopulation of tissue macrophages. Involved in cell-cell interactions, sialoadhesin is structurally related to the 3 other listed members of the sialoadhesin family. CD169 is a sialic acid binding site of sialoadhesin. CD169 is a macrophage receptor expressed on stromal macrophages in many tissues, particularly found in lymph nodes, bone marrow and spleen.Synonyms: Sialic acid-binding Ig-like lectin 1, Sialoadhesin, Siglec-1
- UniProt
- D3ZVM6
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