CRH 抗体
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Quick Overview for CRH 抗体 (ABIN111806)
抗原
See all CRH 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Recognises a different epitope than clone 4H9 (BM357). 5D5 mAb binding decreases only partly after CA treatment. It is possible that the antigen determinant is exposed more openly after DEP modification of histidine residues, whereas this determinant becomes barely accesible after lysine modification. Human serum, containing CRF-binding protein, inhibits the binding of CRF to this antibody.Aff. Const.:1.5 x 10-8M for native CRF, 2 x 10-9M for modified CRF
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纯化方法
- Affinity Chromatography on Protein G
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免疫原
- Synthetic Human Corticotropin Releasing Factor
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亚型
- IgG1
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anti-Corticotropin Releasing Hormone (CRH) (AA 40-195) antibody
CRH 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Corticotropin Releasing Hormone (CRH) (N-Term) antibodyCRH 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Corticotropin Releasing Hormone (CRH) (AA 154-196) antibodyCRH 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2B11 unconjugated
anti-Corticotropin Releasing Hormone (CRH) (AA 39-187) antibodyCRH 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Corticotropin Releasing Hormone (CRH) antibodyMicroarray verified CRH 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB189288 unconjugated
anti-Corticotropin Releasing Hormone (CRH) antibodyMicroarray verified CRH 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB701026 unconjugated
anti-Corticotropin Releasing Hormone (CRH) (AA 185-196) antibodyCRH 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Corticotropin Releasing Hormone (CRH) (AA 25-194) antibodyCRH 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Corticotropin Releasing Hormone (CRH) antibodyMicroarray verified CRH 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB561789 unconjugated
anti-Corticotropin Releasing Hormone (CRH) (AA 28-160) antibodyCRH 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: High binding constant allows this antibody to be used in a sandwich ELISA withclone number 4H9 (BM357), Sensitivity: 25 pg/mL. Immunohistochemistry on frozen sections: A 4 μg/mL solution has worked well on paraffinsections, after microwave pre-treatment.0.1 % trypsin digestion may also be necessary,The detection system used was avidin-biotin. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, 0.09 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- CRH (Corticotropin Releasing Hormone (CRH))
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别名
- Corticoliberin
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背景
- CRF precursor is a 41-amino acid peptide derived from a 196-amino acid preprohormone. CRF is a neuroendocrine hormone secreted by the paraventricular nucleus (PVN) of the hypothalamus and is a key player in the hypothalamic pituitary adrenal axis. It is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. Neuronal CRF is important in a variety of diverse roles including, memory, depression, stress, kidney function, parturition, metabolism and feeding (to mention a few).Synonyms: CRF, CRH, Corticotropin-Releasing Factor, Corticotropin-Releasing Hormone
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基因ID
- 1392
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NCBI登录号
- NP_000747
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UniProt
- P06850
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途径
- Positive Regulation of Peptide Hormone Secretion, Hormone Activity, Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Feeding Behaviour
抗原
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