HCV E1 抗体
HCV E1
适用: Hepatitis C Virus (HCV)
WB, IF, EIA, IHC (fro)
宿主: 小鼠
Monoclonal
BDI198
unconjugated
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- 抗原 See all HCV E1 products
- HCV E1 (Hepatitis C Virus Envelope Protein E1 (HCV E1))
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适用
- Hepatitis C Virus (HCV)
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This HCV E1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 纯化方法
- Protein A Chromatography (Source: Ascites).
- 免疫原
- Recombinant HCV E1 glycoprotein (genotype 1b).
- 克隆位点
- BDI198
- 亚型
- IgG
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- 应用备注
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IFA. ELISA. Western blot. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 2.0 mg/mL (OD280 nm, E0.1% = 1.3)
- 缓冲液
- 0.01 M PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative without stabilizing proteins.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- HCV E1 (Hepatitis C Virus Envelope Protein E1 (HCV E1))
- 别名
- HCV Envelope Glycoprotein E1 (HCV E1 产品)
- 物质类
- Viral Protein
- 背景
- Envelope glycoproteins E1 and E2 are involved in virus attachment to the host cell as well as in virus endocytosis and fusion with host membrane. E2 inhibits human EIF2AK2/PKR activation, preventing the establishment of an antiviral state. E2 is a viral ligand for CD209/DC-SIGN and CLEC4M/DC-SIGNR, which are respectively found on dendritic cells (DCs), and on liver sinusoidal endothelial cells and macrophage-like cells of lymph node sinuses. These interactions allow capture of circulating HCV particles by these cells and subsequent transmission to permissive cells. DCs are professional antigen presenting cells, critical for host immunity by inducing specific immune responses against a broad variety of pathogens. They act as sentinels in various tissues where they entrap pathogens and convey them to local lymphoid tissue or lymph node for establishment of immunity. Capture of circulating HCV particles by these SIGN+ cells may facilitate virus infection of proximal hepatocytes and lymphocyte subpopulations and may be essential for the establishment of persistent infection.Synonyms: Heptitis C Virus E1, gp32, gp35
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