CD9 抗体 (FITC)
Quick Overview for CD9 抗体 (FITC) (ABIN1112243)
抗原
See all CD9 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
- Monoclonal Mouse Anti-Human CD9F is recommended for use in flow cytometry for identification of human platelets.
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亚型
- IgG2a
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anti-CD9 (CD9) (AA 112-195) antibody
CD9 适用: 人 FACS, WB, IHC, ELISA, IF 宿主: 小鼠 Monoclonal 1E8H1 unconjugated
anti-CD9 (CD9) (AA 112-195) antibodyCD9 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 5A6D8 unconjugated
anti-CD9 (CD9) antibodyCD9 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal CD9-1619 unconjugated
anti-CD9 (CD9) antibodyCD9 适用: 人 IHC, StM, ISt 宿主: 小鼠 Monoclonal CD9-2343 unconjugated
anti-CD9 (CD9) (Internal Region) antibodyCD9 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD9 (CD9) antibodyCD9 适用: 人 FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 5G6 unconjugated
anti-CD9 (CD9) (AA 101-200) antibodyCD9 适用: 人, 小鼠, Cow, 大鼠, Pig FACS, WB, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD9 (CD9) antibody (Biotin)CD9 适用: 小鼠 FACS, WB, IP, ICC 宿主: 大鼠 Monoclonal EM-04 Biotin
anti-CD9 (CD9) antibodyCD9 适用: 人 FACS, WB, IHC (p), MS 宿主: 小鼠 Monoclonal MEM-61 unconjugated
anti-CD9 (CD9) (AA 115-145) antibodyCD9 适用: 人 FACS, WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.
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说明
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Fluorescein isothiocyanate (Molecular Probes)
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样品收集
- 1. Transfer 5 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add 95 µl of PBS (It better that it containing 2% bovine serum albumin) without Fixative mix gently with a vortex mixer. 3. Add 20 µl of CD9 FITC and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 4. The recommended negative control is a non-reactive FITC-conjugated antibody of the same isotype. 5. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 6. Add 0,2 ml 0.01 mol/l PBS ( It better that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.
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储存液
- Sodium azide
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注意事项
- 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. Analyse on a flow cytometer, and the readings of the cytometer are recorded, bearing in the mind that the FSC and SSC light dispersion parameters must be on a logarithmic scale. 3. As with any product derived from biological sources, proper handling procedures should be used.
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储存条件
- 4 °C
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- CD9
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别名
- CD9
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背景
- This antibody reacts with a 25 kDa molecular weight. The antigen is expressed on eosinophils, granulocytes, monocytes and numerous stromal cells. This CD9 antigen belongs to the TM4 superfamily of cell surface proteins with the N- and C-terminals cytoplasmically located. This antibody reacts immunohistochemically on frozen sections of platelets, monocytes and pre-B cells with differential staining on lymphoid and epithelial tissues.
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途径
- Response to Water Deprivation, Cell-Cell Junction Organization
抗原
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