DPP4 抗体 (FITC)
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- 抗原 See all DPP4 抗体
- DPP4 (Dipeptidyl-Peptidase 4 (DPP4))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This DPP4 antibody is conjugated to FITC
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF)
- 产品特性
- Monoclonal Mouse Anti-Human CD26 FITC is recommended for use in flow cytometry for identification of human receptor for Interleukin-2 (IL-2R) expressing the 55,000 M.W. surface antigen.
- 克隆位点
- TP1-19
- 亚型
- IgG2b
- Top Product
- Discover our top product DPP4 Primary Antibody
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anti-Dipeptidyl-Peptidase 4 (DPP4) (AA 484-728) antibody
DPP4 适用: 人 WB, IHC (fro), ELISA, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Dipeptidyl-Peptidase 4 (DPP4) (AA 631-760) antibodyDPP4 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dipeptidyl-Peptidase 4 (DPP4) (AA 29-178) antibodyDPP4 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dipeptidyl-Peptidase 4 (DPP4) (AA 473-588) antibodyDPP4 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Dipeptidyl-Peptidase 4 (DPP4) (Internal Region) antibodyVerified DPP4 适用: 人 FACS, WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Dipeptidyl-Peptidase 4 (DPP4) antibody (PE)DPP4 适用: 人 FACS 宿主: 小鼠 Monoclonal BA5b PE
anti-Dipeptidyl-Peptidase 4 (DPP4) antibodyDPP4 适用: 人, 大鼠, 小鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Dipeptidyl-Peptidase 4 (DPP4) (Internal Region) antibodyDPP4 适用: 人, Cow, Pig, 马, 猴, 兔, Bat IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Dipeptidyl-Peptidase 4 (DPP4) (C-Term) antibodyDPP4 适用: 人, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Dipeptidyl-Peptidase 4 (DPP4) antibody (FITC)DPP4 适用: 人 FACS 宿主: 小鼠 Monoclonal BA5b FITC
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- 应用备注
- It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.
- 说明
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Fluorescein isothiocyanate (Molecular Probes).
- 样品收集
- 1. Transfer 100 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add 20 µl of CD26 FITC and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive FITC-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 100 µl of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 ml 0.01 mol/l PBS ( It better that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 ml PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.
- 储存液
- Sodium azide
- 注意事项
- 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.
- 储存条件
- 4 °C
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- 抗原
- DPP4 (Dipeptidyl-Peptidase 4 (DPP4))
- 别名
- CD26 (DPP4 产品)
- 别名
- ADABP antibody, ADCP2 antibody, CD26 antibody, DPPIV antibody, TP103 antibody, MGC81966 antibody, MOP9.8 antibody, MOP9_8 antibody, DPP4 antibody, si:ch73-2d23.3 antibody, Cd26 antibody, Dpp-4 antibody, THAM antibody, dipeptidyl peptidase 4 antibody, dipeptidyl-peptidase 4 S homeolog antibody, prolyl oligopeptidase family protein antibody, dipeptidyl-peptidase 4 antibody, dipeptidylpeptidase 4 antibody, DPP4 antibody, dpp4.S antibody, AT5G24260 antibody, dpp4 antibody, Dpp4 antibody
- 背景
- The monoclonal antibody is directed against the CD26- antigen, which is expressed on human activated T- and B- cells. CD26 is required for T cell proliferation. It is expressed on 10-60% of resting T cells in normal peripheral blood. There is a selective decrease of CD4+ CD26+ T cells in HIV-1 infected individuals.
- 途径
- Peptide Hormone Metabolism, Regulation of Leukocyte Mediated Immunity
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