(1 reference)-
- 抗原 See all CD235a/GYPA (GYPA) 抗体
- CD235a/GYPA (GYPA) (Glycophorin A (GYPA))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD235a/GYPA antibody is conjugated to FITC
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF)
- 产品特性
- Monoclonal Mouse Anti-Human CD235a FITC is recommended for use in flow cytometry.
- 克隆位点
- HI264
- 亚型
- IgG2a
- Top Product
- Discover our top product GYPA Primary Antibody
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anti-Glycophorin A (GYPA) (AA 20-91) antibody
GYPA 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glycophorin A (GYPA) (AA 1-108) antibodyGYPA 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glycophorin A (GYPA) antibodyGYPA 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal GYPA-280 unconjugated
anti-Glycophorin A (GYPA) (Internal Region) antibodyGYPA 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Glycophorin A (GYPA) antibody (FITC)GYPA 适用: 人, 大鼠 FACS 宿主: 小鼠 Monoclonal JC159 FITC
anti-Glycophorin A (GYPA) antibody (PE)GYPA 适用: 人, 大鼠 FACS 宿主: 小鼠 Monoclonal JC159 PE
anti-Glycophorin A (GYPA) antibodyGYPA 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal rGYPA-280 unconjugated Recombinant Antibody
anti-Glycophorin A (GYPA) antibodyGYPA 适用: 人, 大鼠 FACS, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal JC159 unconjugated
anti-Glycophorin A (GYPA) (C-Term) antibodyVerified GYPA 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Glycophorin A (GYPA) (C-Term) antibodyGYPA 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.
- 说明
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Fluorescein isothiocyanate (Molecular Probes) Anti-CD235a HI264 was included in the Seventh International Workshops on Human Leucocyte Differentiation Antigens.
- 样品收集
- 1. Transfer 5 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add 95 µl of PBS (It better that it containing 2% bovine serum albumin) without Fixative mix gently with a vortex mixer. 3. Add 20 µl of CD235a FITC and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 4. The recommended negative control is a non-reactive FITC-conjugated antibody of the same isotype. 5. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 6. Add 0, 2 ml 0.01 mol/l PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 7. Analyse on a flow cytometer, and the readings of the cytometer are recorded, bearing in the mind that the FSC and SSC light dispersion parameters must be on a logarithmic scale.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.
- 储存液
- Sodium azide
- 注意事项
- 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.
- 储存条件
- 4 °C
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: "Immunophenotyping investigation of minimal residual disease is a useful approach for predicting relapse in acute myeloid leukemia patients." in: Blood, Vol. 90, Issue 6, pp. 2465-70, (1997) (PubMed).
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: "Immunophenotyping investigation of minimal residual disease is a useful approach for predicting relapse in acute myeloid leukemia patients." in: Blood, Vol. 90, Issue 6, pp. 2465-70, (1997) (PubMed).
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- 抗原
- CD235a/GYPA (GYPA) (Glycophorin A (GYPA))
- 别名
- CD235a (GYPA 产品)
- 途径
- Maintenance of Protein Location
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