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TUBB3 抗体 (N-Term) (FITC)

TUBB3 适用: Vicia faba WB, FACS, IHC (p), IF, IHC (fro), EIA 宿主: 小鼠 Monoclonal TU-20 FITC
产品编号 ABIN110981
发货至: 中国
  • 抗原 See all TUBB3 抗体
    TUBB3 (Tubulin, beta 3 (TUBB3))
    抗原表位
    • 21
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    N-Term
    适用
    • 127
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    • 21
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    Vicia faba
    宿主
    • 81
    • 49
    • 4
    • 1
    • 1
    小鼠
    克隆类型
    • 71
    • 65
    单克隆
    标记
    • 82
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    This TUBB3 antibody is conjugated to FITC
    应用范围
    • 110
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    Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
    特异性
    This antibody recognizes the C-terminal peptide sequence ESESQGPK (aa 441-448) of human class III β-tubulin specific for neurones. The antibody is a highly specific marker for neuronal tissue. TU-20 is very useful for the detection of microtubule structures on fixed cells. MAb TU-20 is widely cross-reactive among species (recognized epitope conserved within all species).
    免疫原
    Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide
    克隆位点
    TU-20
    亚型
    IgG1
    Top Product
    Discover our top product TUBB3 Primary Antibody
  • 应用备注
    Immunohistochemistry on Frozen and Paraffin Sections. Immunocytotochemistry on Fixed and Permeabilized cells. Suggested working dilution is 1/40. The conjugate was also successfully used on FFPE Sections using Confocal Microscopy.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 浓度
    1.0 mg/mL
    缓冲液
    PBS containing 15 mM Sodium Azide as preservative, approx. pH 7.4
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody at 2-8 °C for one month or at -20 °C for longer.
  • Katsetos, Draber, Kavallaris: "Targeting βIII-tubulin in glioblastoma multiforme: from cell biology and histopathology to cancer therapeutics." in: Anti-cancer agents in medicinal chemistry, Vol. 11, Issue 8, pp. 719-28, (2012) (PubMed).

    Theodorou, Dalembert, Heffelfinger, White, Weissman, Corcoran, Snyder: "A high throughput embryonic stem cell screen identifies Oct-2 as a bifunctional regulator of neuronal differentiation." in: Genes & development, Vol. 23, Issue 5, pp. 575-88, (2009) (PubMed).

    Dráberová, Del Valle, Gordon, Marková, Smejkalová, Bertrand, de Chadarévian, Agamanolis, Legido, Khalili, Dráber, Katsetos: "Class III beta-tubulin is constitutively coexpressed with glial fibrillary acidic protein and nestin in midgestational human fetal astrocytes: implications for phenotypic identity." in: Journal of neuropathology and experimental neurology, Vol. 67, Issue 4, pp. 341-54, (2008) (PubMed).

  • 抗原
    TUBB3 (Tubulin, beta 3 (TUBB3))
    别名
    TUBB3 / TUBB4 (TUBB3 产品)
    别名
    CDCBM antibody, CFEOM3A antibody, TUBB4 antibody, beta-4 antibody, TUBB3 antibody, 3200002H15Rik antibody, M(beta)3 antibody, M(beta)6 antibody, tubb3 antibody, tubulin beta 3 class III antibody, tubulin, beta 3 class III antibody, tubulin beta 3 class III L homeolog antibody, beta-tubulin 3 antibody, tubulin beta-3 chain antibody, beta tubulin 3 antibody, TUBB3 antibody, Tubb3 antibody, tubb3 antibody, tubb3.L antibody, LOC100581245 antibody, tub3 antibody
    背景
    The betaIII-tubulin isotype is present dominantly in cells of neuronal origin and it is one of the earliest marker of neuronal differentiation. Class III beta-tubulin, is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The neuron-associated class III beta-tubulin isotype is most abundant in cells of neuronal origin but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.Synonyms: Tubulin beta-3 chain, Tubulin beta-4, Tubulin beta-III
    基因ID
    10381
    NCBI登录号
    NP_001184110
    UniProt
    Q13509
    途径
    Microtubule Dynamics, M Phase
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