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Toxoplasma Gondii P30 抗体

This 小鼠 单克隆 antibody specifically detects Toxoplasma Gondii P30 in WB, EIA 和 IF. It exhibits reactivity toward Toxoplasma gondii.
产品编号 ABIN110947
发货至: 中国
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Quick Overview for Toxoplasma Gondii P30 抗体 (ABIN110947)

抗原

Toxoplasma Gondii P30

适用

  • 5
  • 2
Toxoplasma gondii

宿主

  • 6
  • 1
小鼠

克隆类型

  • 7
单克隆

标记

  • 7
非结合性

应用范围

  • 5
  • 4
  • 2
  • 2
  • 1
  • 1
Western Blotting (WB), Enzyme Immunoassay (EIA), Immunofluorescence (IF)

克隆位点

P30-3
  • 纯化方法

    Affinity Chromatography on Protein G

    免疫原

    Native Toxoplasma gondii p30 (purified).

    亚型

    IgG2a
  • 应用备注

    ELISA: As a guide, the end point titre in an indirect ELISA has been found to be1/2,000,000 using purified T. gondii P30 as the coating antigen. Western Blot.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    PBS buffer, pH 7.2 containing 0.09 % Sodium Azide as preservative

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原

    Toxoplasma Gondii P30

    背景

    Toxoplasma gondii p30 is the immunodominant surface antigen of Toxoplasma gondii. Toxoplasma gondii is an obligate intracellular protozoan that belongs to the class Sporozoa, and the order Eucoccidia. The parasite has an extremely wide host range and is also nonspecific with respect to the kind of cell type it can enter, making it an interesting model to study invasion. Although toxoplasmosis in healthy adults is generally asymptomatic, it produces severe disease in neonates and immunocompromised people, being the cause of death in many AIDS patients. P30 is the major surface antigen of T. gondii. It is a very abundant protein, conserved in most strains, and present both in the vesicular network of the parasitophorous vacuole and on the surface of the parasite. It is very immunogenic, eliciting high titers of antibodies in infected individuals. The gene coding for P30 has been cloned and completely sequenced: the deduced amino acid sequence shows a putative signal sequence at the N terminus, consistent with localization of P30 on the parasite's surface, as well as a short hydrophobic stretch at the C terminus.Synonyms: Major surface antigen p30

    基因ID

    5811

    UniProt

    P13664
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