Specificity has been tested in ELISA. In immunoblotting, under less stringent washing conditions, a weaker band of approximately 60 kDa is also detected (Figure 1, Right Panel). Additional tests for cross reactivity have not yet been performed.
交叉反应 (详细)
Species reactivity (tested):Human.
纯化方法
Purified
免疫原
Recombinant GST-hRPP20 (NCBI accession number AAC24113, expression vector pGEC-2T), expressed in E.coli.
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
溶解方式
Dissolve in 200 μL buffer for a 0.5 mg/mL antibody concentration in 1 % BSA
浓度
0.5 mg/mL (when dissolved at 0.5 mg/mL, the BSA concentration will be 1%)
缓冲液
10 mM Ammonium Bicarbonate buffer, 2 mg BSA
注意事项
Be careful opening the vial since the antibody resides in a Vacuum.
储存条件
4 °C
储存方法
Store the antibody undiluted at 2-8 °C or Add up to 0.05% Sodium Azide for long term storage.
抗原
POP7
(Processing of Precursor 7, Ribonuclease P/MRP Subunit (POP7))
别名
POP7
背景
The nuclei of eukaryotic cells contain several classes of small RNA-protein complexes. SnRNPs (small nuclear ribonucleoproteins) are involved in splicing of pre-mRNAs (the excision of introns) in the nucleoplasm. RPP20 is part of such a complex. SnoRNPs (small nucleolar ribonucleoproteins) are involved in the maturation of pre-ribosomal RNA in the nucleoli. Pre-rRNA processing and modification require both the snoRNA and the protein constituents (such as Rpp20) of these complexes. Generally, snoRNPs contain a number of common proteins, which are shared with other snoRNPs of the same family, next to several particle-specific proteins. On average, snoRNPs contain about 6-10 protein subunits.Synonyms: RPP20, Ribonuclease P protein subunit p20, Ribonucleases P/MRP protein subunit POP7 homolog, hPOP7