The antibody is specific to extradomain A (EDA) sequence of a Cellular Fibronectin and recognizes thus only the Cellular Fibronectin (cFN). Clone DHI has been shown to specifically block Chondrocyte condensation in Chicken embryos (ref. 5).
交叉反应 (详细)
Species reactivity (tested):Human, Chicken, Rat, Rabbit and Guinea Pig.
纯化方法
Ig Fraction
免疫原
Purified Fibronectin from A8387 fibrosarcoma cells (ref. 21). Hybridoma produced by fusion between myeloma cells and Balb/c spleen cells.
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
浓度
0.1 mg/mL
缓冲液
PBS, 0.09 % (w/v) Sodium Azide, 1.0 % (w/v) BSA
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C
储存方法
Store the antibody undiluted at 2-8 °C.
抗原
Fibronectin
背景
Fibronectins are diverse high molecular weight cell adhesion glycoproteins found in basement membranes, in the interstitial connective tissue matrix, and in soluble in plasma and other body fluids. In adult tissues most fibronectin is produced by hepatocytes (pFn) that differ from locally produced cellular fibronectins. A monoclonal antibody (AM32091PU-N Clone BF12) has been produced to fibronectin that reacts with both the cellular and plasma forms of the protein and recognizes the cell-binding region in fibronectin. The monoclonal antibody DH1 recognizes only cellular form of Fibronectin. DH1 is specific to the extra domain A (EDA) of the molecule. cFns are found in some basement membranes during development and only in distinct tissues in adult, for instance in smooth muscle and alveolar walls of airways. cFns are abundant in the plasma of carcinoma patients and in the stroma of various carcinomas.Synonyms: CIG, Cold-insoluble globulin, FN1