eEF1A1 抗体 (N-Term)
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Quick Overview for eEF1A1 抗体 (N-Term) (ABIN1107053)
抗原
See all eEF1A1 (EEF1A1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- N-Term
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特异性
- The monoclonal antibody 900 recognizes elongation factor Tu (EF-Tu), a 43 kDa multifunctional protein present in Escherichia coli. The antibody recognizes EF-Tu in organisms belonging to bacterial and archaeal domains, yet no organisms from the eukaryotic domain. The panbacterial distribution of EF-Tu, which is present in large amounts in every prokaryotic cell, renders this protein a good candidate for diagnostic purposes. The highly conserved epitope recognized by monoclonal antibody 900 is located at the very end of the N-terminus of the EF-Tu molecule (SKEKFE).
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交叉反应 (详细)
- Species reactivity (tested):E. coli. Cross react with Bacteroides fragilis, Streptococcus oralis, Bacillus subtilis, Pseudomonas aeruginosa, Burkholderia cepacia, and Deinococcus sp., weakly cross reacts with Mycobacterium tuberculosis.
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纯化方法
- Protein G purified
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免疫原
- E. coli K-12 C600 Rif ( pKT146) bacteria
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亚型
- IgG2a
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.02 % sodium azide, 0.1 % bovine serum albumin
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Product should be stored at 2-8 °C.
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- eEF1A1 (EEF1A1) (Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1))
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别名
- EEF1A1 / LENG7
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背景
- EF-Tu is one of the most abundant proteins present in prokaryotes, representing about 5 % of the total cellular protein of E. coli. During protein biosynthesis, the elongation process, EF-Tu catalyzes the binding of each aminoacyl-tRNA to the ribosome. It also interacts with several macromolecules and guanine nucleotides, including EF-Ts, GDP, GTP, and some ribosomal proteins.Synonyms: CCS-3, CCS3, EEF1A, EF-1-alpha-1, EF-Tu, EF1A, Elongation factor 1 alpha 1, Elongation factor Tu, Eukaryotic elongation factor 1 A-1, FLJ25721, GRAF-1EF, Leukocyte receptor cluster member 7, PTI1, eEF1A-1
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基因ID
- 1915
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NCBI登录号
- NP_001393
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UniProt
- P68104
抗原
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