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eEF1A1 抗体 (N-Term)

The 小鼠 单克隆 anti-eEF1A1 antibody is suitable to detect eEF1A1 in samples from Bacteria. It has been validated for WB, IF 和 EIA.
产品编号 ABIN1107053
发货至: 中国
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Quick Overview for eEF1A1 抗体 (N-Term) (ABIN1107053)

抗原

See all eEF1A1 (EEF1A1) 抗体
eEF1A1 (EEF1A1) (Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1))

适用

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Bacteria

宿主

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小鼠

克隆类型

  • 61
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单克隆

标记

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This eEF1A1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

克隆位点

9E2
  • 抗原表位

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    N-Term

    特异性

    The monoclonal antibody 900 recognizes elongation factor Tu (EF-Tu), a 43 kDa multifunctional protein present in Escherichia coli. The antibody recognizes EF-Tu in organisms belonging to bacterial and archaeal domains, yet no organisms from the eukaryotic domain. The panbacterial distribution of EF-Tu, which is present in large amounts in every prokaryotic cell, renders this protein a good candidate for diagnostic purposes. The highly conserved epitope recognized by monoclonal antibody 900 is located at the very end of the N-terminus of the EF-Tu molecule (SKEKFE).

    交叉反应 (详细)

    Species reactivity (tested):E. coli. Cross react with Bacteroides fragilis, Streptococcus oralis, Bacillus subtilis, Pseudomonas aeruginosa, Burkholderia cepacia, and Deinococcus sp., weakly cross reacts with Mycobacterium tuberculosis.

    纯化方法

    Protein G purified

    免疫原

    E. coli K-12 C600 Rif ( pKT146) bacteria

    亚型

    IgG2a
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    限制

    仅限研究用
  • 浓度

    0.1 mg/mL

    缓冲液

    PBS, 0.02 % sodium azide, 0.1 % bovine serum albumin

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Product should be stored at 2-8 °C.
  • 抗原

    eEF1A1 (EEF1A1) (Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1))

    别名

    EEF1A1 / LENG7

    背景

    EF-Tu is one of the most abundant proteins present in prokaryotes, representing about 5 % of the total cellular protein of E. coli. During protein biosynthesis, the elongation process, EF-Tu catalyzes the binding of each aminoacyl-tRNA to the ribosome. It also interacts with several macromolecules and guanine nucleotides, including EF-Ts, GDP, GTP, and some ribosomal proteins.Synonyms: CCS-3, CCS3, EEF1A, EF-1-alpha-1, EF-Tu, EF1A, Elongation factor 1 alpha 1, Elongation factor Tu, Eukaryotic elongation factor 1 A-1, FLJ25721, GRAF-1EF, Leukocyte receptor cluster member 7, PTI1, eEF1A-1

    基因ID

    1915

    NCBI登录号

    NP_001393

    UniProt

    P68104
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