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- 抗原
- Complex IV Subunit I
- 抗原表位
- AA 2-14, N-Term
- 适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB)
- 特异性
- This antibody detects Complex IV subunit I (N-term).
- 交叉反应 （详细）
- Species reactivity (tested):Human
- 纯化方法
- Immunogen affinity purified
- 免疫原
- A synthetic peptide corresponding to a sequence at the N-terminal of human CO1 (2-14 aa, FADRWLFSTNHKD) .Remarks: Sequence is identical to the related rat and mouse sequence.
- 亚型
- IgG
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anti-Complex IV Subunit I (AA 202-232), (Middle Region) antibody
适用: 人 WB, EIA, FACS 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 溶解方式
- 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/ml.
- 缓冲液
- 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3
- 储存液
- Sodium azide, Thimerosal (Merthiolate)
- 注意事项
- This product contains Sodium azide and Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
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- 抗原
- Complex IV Subunit I
- 背景
- Cytochrome c oxidase subunit I (CO1 or MTCO1) is 1 of 3 mitochondrial DNA (mtDNA) encoded subunits (MTCO1, MTCO2, MTCO3) of respiratory Complex IV. Complex IV is located within the mitochondrial inner membrane and is the third and final enzyme of the electron transport chain of mitochondrial oxidative phosphorylation. It is composed of 13 polypeptides. Subunits I, II, and III (MTCO1, MTCO2, MTCO3) are encoded by mtDNA while subunits IV, Va, Vb, VIa, VIb, VIc, VIIa, VIIb,VIIc, and VIII are nuclear encoded.1,2 The cytochrome c oxidase family of enzymes have 4 redox centers ,2 hemes and 2 copper centers. In mitochondrial Complex IV, the 2 hemes are a and a3 and the 2 coppers are CuA and CuB. The 2 hemes and CuB are bound to subunit I.3 Acin-Perez et al. (2003)identified a cell line containing single and double missense mutations in the cytochrome c oxidase (COX)subunit I gene of mouse mitochondrial DNA. And they hypothesized that deleterious mutations can arise and become predominant, cultured cells can maintain several mtDNA haplotypes at stable frequencies, the respiratory chain has little spare COX capacity, and that the size of a cavity in the vicinity of val421 in MTCO1I of animal COX may affect the function of the enzyme.4Synonyms: COI, COXI, Cytochrome c oxidase subunit 1, MT-CO1, MTCO1, Mitochondria Complex IV (Cytochrome C Oxidase)
- 基因ID
- 4512
- NCBI登录号
- AP_000641
- UniProt
- P00395
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