CNF1 (AA 704-730) 抗体
适用: E. coli
WB, Func, DB, EIA
宿主: 小鼠
Monoclonal
NG8
unconjugated
-
- 抗原
- CNF1
- 抗原表位
- AA 704-730
-
适用
- E. coli
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- 非结合性
- 应用范围
- Western Blotting (WB), Functional Studies (Func), Dot Blot (DB), Enzyme Immunoassay (EIA)
- 特异性
- The Monoclonal antibody clone NG8 recognizes an epitope between amino acids 704 and 730 of the C-terminal enzymatic domain. NG8 specifically neutralizes CNF1 while lacking activity for CNF2.
- 免疫原
- Synthetic peptide, corresponding to amino acids 704/730 of Escherichia coli CNF1
- 克隆位点
- NG8
- 亚型
- IgG2a
-
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
-
- 浓度
- 0.1 mg/mL
- 缓冲液
- 0.1 % BSA
- 储存条件
- 4 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C.
-
- 抗原
- CNF1
- 背景
- CNF1 and CNF2 belong to a family of bacterial toxins that target the small GTP-binding Rho proteins that regulate the actin cytoskeleton. Members of this toxin family typically inactivate Rho, however, CNF1 and the highly related CNF2 activate Rho by deamidation. CNF1 is more frequently associated with E.coli strains that cause extraintestitinal infections in humans, particularly those of the urinary tract (such as cystitis, pyelonephritis and prostatitis). In CNF1-producing uropathogenic E. coli strains, CNF1 is chromosomally encoded and typically resides on a pathogenicity island that also contains hemolysin and P fimbria- related genes. Both CNF1 and the highly related, plasmid-encoded CNF2 are monomeric, cytoplasmic toxins of approximately 115 kDa. CNF1 can be structurally organized into three functional domains the N-terminal, central and the C-terminal domain. The latter exhibits the catalytic activity of the toxin.Synonyms: Cytotoxic necrotizing factor 1
- 分子量
- 115 kDa
- 基因ID
- 3990770
- NCBI登录号
- YP_543855
- UniProt
- Q1R2U0
-
You are here:
