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- 抗原 See all CD43 (SPN) 抗体
- CD43 (SPN) (Sialophorin (SPN))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD43 antibody is conjugated to FITC
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应用范围
- Flow Cytometry (FACS)
- 特异性
- This antibody reacts with CD43 antigen. Clone 1D4 selectively recognizes core 2-containing O-glycans on CD43, which have the GlcNAc1->6 branch attached to the C-6 position of GalNAc and are formed by core 2 -1,6-N-acetylglucosaminyltransferase (C2GnT). This type of CD43 is preferentially expressed in the CD45RO+ memory subset of CD4+ cells.
- 交叉反应 （详细）
- Species reactivity (tested):Human
- 纯化方法
- Protein A agarose
- 免疫原
- PHA-activated human T cell line maintained with IL-2 containing media
- 克隆位点
- 1D4
- 亚型
- IgG2a
- Top Product
- Discover our top product SPN Primary Antibody
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anti-Sialophorin (SPN) antibody
SPN 适用: 人 FACS, WB, IHC, IF, StM 宿主: 小鼠 Monoclonal DF-T1 unconjugated
anti-Sialophorin (SPN) antibodySPN 适用: 人 FACS, WB, IHC (p), IP 宿主: 小鼠 Monoclonal MEM-59 unconjugated
anti-Sialophorin (SPN) antibodySPN 适用: 人 FACS, WB, IHC (p), IP, Func 宿主: 小鼠 Monoclonal MEM-59 unconjugated
anti-Sialophorin (SPN) (AA 276-400) antibodySPN 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Sialophorin (SPN) antibodySPN 适用: 人 FACS, WB, IHC, IF, StM 宿主: 兔 Monoclonal SPN-1766R unconjugated Recombinant Antibody
anti-Sialophorin (SPN) antibodySPN 适用: 人 FACS, WB, IHC, IF, StM 宿主: 小鼠 Monoclonal rSPN-839 unconjugated Recombinant Antibody
anti-Sialophorin (SPN) antibodySPN 适用: 人, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Sialophorin (SPN) (AA 1-400) antibodySPN 适用: 人 FACS, WB 宿主: 小鼠 Polyclonal unconjugated
anti-Sialophorin (SPN) antibody (FITC)SPN 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-59 FITC
anti-Sialophorin (SPN) antibody (Biotin)SPN 适用: 人 FACS, WB, IHC (p), IP 宿主: 小鼠 Monoclonal MEM-59 Biotin
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 缓冲液
- NaPB ( pH 8.0) containing 1 % BSA, 150 mM NaCl and 0.09 % NaN3, 1 % BSA
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store at 2 - 8 °C.
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- 抗原
- CD43 (SPN) (Sialophorin (SPN))
- 别名
- CD43 / Leukosialin (SPN 产品)
- 别名
- CD43 antibody, GALGP antibody, GPL115 antibody, LSN antibody, A630014B01Rik antibody, Cd43 antibody, Galgp antibody, Ly-48 antibody, Ly48 antibody, Lsn antibody, Lsn1 antibody, gpL115 antibody, sialophorin antibody, SPN antibody, Spn antibody
- 背景
- CD43 antigen is a heavily sialylated single chain membrane glycoprotein with both molecular weight and antigenicity depending on the nature and complexity of the attached O-linked polysaccharides. CD43 is an integral membrane protein with extracellular domain (235 aa), transmembrane domain (23 aa) and intracytoplasmic domain (123 aa). The extracellular domain of CD43 has been shown to react with many ligand such as ICAM-1 (CD54), E-selectin, galectin-1, MHC-I and human serum albumin (HSA). The intracytoplasmic domain, which is highly conserved between species, is constitutively phosphorylated on serine residues. The level of phosphorylation increases after cell activation. This cell surface glycoprotein is also called leukosialin, sialophorin or GPL115. A soluble form of CD43 is also present in human serum. Thymocytes, CD4+ T lymphocytes and monocytes express more of a 115 kDa CD43 isoform, whereas a 130 kDa isoform is found mostly on activated CD4+ T cells, CD8+ resting and activated T cells, neutrophils, platelets and B cells. CD43 plays a role in the regulation of lymphoid cell adhesion, cellular maturation and activation although the nature of this participation remains controversial. For example, the structural characteristics of the CD43 extracellular domain indicate that this molecule has both adhesive and anti-adhesive properties. O-glycosylated chains of CD43 such as a sialyl Lewis X (sLex) epitope, a ligand for both P- and E-selectin, potentiate binding to lectin-like molecules. However, the adhesion molecule function for CD43 is counteracted by the presence of negatively charged sialic acid residues on its extracellular domain. In addition to the strong structural evidence for the involvement of CD43 in cell interactions, experimental data suggest contradictive role for CD43 in regulating cell contacts.Synonyms: Galactoglycoprotein, Leukocyte sialoglycoprotein, SPN, Sialophorin
- 基因ID
- 6693
- NCBI登录号
- NP_001025459
- UniProt
- P16150
- 途径
- Regulation of Leukocyte Mediated Immunity
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