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Actin 抗体 (C-Term, pan)

This anti-Actin antibody is a 小鼠 单克隆 antibody detecting Actin in WB, IF 和 IHC (fro). Suitable for 人, 大鼠, 小鼠, 小鸡 和 Snake.
产品编号 ABIN1105234
发货至: 中国

Quick Overview for Actin 抗体 (C-Term, pan) (ABIN1105234)

抗原

See all Actin 抗体
Actin

适用

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人, 大鼠, 小鼠, 小鸡, Snake

宿主

  • 88
  • 41
  • 3
  • 1
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  • 1
小鼠

克隆类型

  • 80
  • 55
单克隆

标记

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This Actin antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

克隆位点

AC-40
  • 抗原表位

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    • 11
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    • 1
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    C-Term, pan

    纯化方法

    Goat anti-Mouse IgG Affinity Chromatography

    免疫原

    Synthetic Actin C-terminal peptide Ser-Gly-Pro-Ser-Ile-Val-His-Arg-Lys-Cys-Phe, attached to a Multiple Antigen Peptide (MAP) backbone.

    亚型

    IgG2a
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    限制

    仅限研究用
  • 溶解方式

    Restore with 1.2 % Sodium Acetate or neutral PBS.

    浓度

    0.1 mg/mL

    缓冲液

    1.2 % Sodium Acetate containing 2 mg BSA and 0.01 mg Sodium Azide as preservative.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody at -20 °C. After reconstituion store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原

    Actin

    背景

    Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90 % sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin,two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins,this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.
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