ZIC2 抗体 (AA 520-531)
Quick Overview for ZIC2 抗体 (AA 520-531) (ABIN1105113)
抗原
See all ZIC2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 520-531
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特异性
- Human ZIC2. This antibody is expected to NOT cross-react with other ZIC proteins.
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预测反应
- Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Bat, Elephant, Opossum, Guinea pig (100%) Galago, Tamarin, Bovine, Dog, Hamster, Panda, Porcine, Xenopus, Medaka, Seabass, Pufferfish, Zebrafish, Stickleback (92%) Zebra finch, Chicken, Lizard, Salmon (83%).
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纯化方法
- Immunoaffinity purified
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免疫原
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Synthetic peptide C-HSGLSSNFNEWY from the C-terminus of human ZIC2 (NP_009060.2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Bat, Elephant, Opossum, Guinea pig (100%), Galago, Tamarin, Bovine, Dog, Hamster, Panda, Porcine, Xenopus, Medaka, Seabass, Pufferfish, Zebrafish, Stickleback (92%), Zebra finch, Chicken, Lizard, Salmon (83%).
Type of Immunogen: Synthetic peptide
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应用备注
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Approved: Peptide-ELISA (1:1000), WB (0.5 - 2 μg/mL)
Usage: Peptide ELISA: antibody detection limit dilution 1:1000. Western blot: In transfected RWPE1 transiently expressing Human ZIC2-Myc a band of approx. 65kD is observed. This band is not observed in the non-transfected RWPE1, nor when GFP is transiently expressed. The calculated molecular size is 55.0kD according to NP_009060.2 and 58.6kD for the ZIC2-Myc protein. Data provided by Nathan Bowen and Maryam Boseman, Clark Atlanta University, GA, USA. We have observed an approx 75kD band in Human Brain (Cerebellum and Cerebral Cortex) lysates Recommended concentration, 0.5-2 μg/mL. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- Tris-buffered saline, pH 7.3, 0.5 % BSA, 0.02 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store at -20°C. Avoid freeze-thaw cycles.
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- ZIC2
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别名
- ZIC2
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背景
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Name/Gene ID: ZIC2
Synonyms: ZIC2, HPE5, Zic family member 2, Zinc finger protein ZIC 2 -
基因ID
- 7546
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NCBI登录号
- NP_009060
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UniProt
- O95409
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途径
- Tube Formation
抗原
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