PLP1 抗体 (C-Term)
Quick Overview for PLP1 抗体 (C-Term) (ABIN1103681)
抗原
See all PLP1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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品牌
- IHC-plus™
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特异性
- Reacts with myelin proteolipid protein in many mammalian species. Shows cross-reactivity to DM20.
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纯化方法
- Purified
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免疫原
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Myelin proteolipid protein, synthetic peptide CGRGTKF corresponding to the C-terminal peptide.
Type of Immunogen: Synthetic peptide -
亚型
- IgG2a
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应用备注
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Approved: ICC, IHC, IHC-Fr, IHC-P (10 μg/mL), WB
Usage: Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Western Blot: Can aggregate causing HMW bands to sometimes appear in certain preparations. Immunocytochemistry: 4 % PFA fixed cells for 5-10 minutes at RT. Use 0.1 % triton in block only to allow antibody access to C-terminal epitope. Immunohistochemistry (Frozen): Fresh frozen sections or 4 % PFA fixed, frozen tissues. Epitope is internal, use 0.1-0.2 % triton X-100 in the blocking step for permeabilization. Immunohistochemistry (Paraffin): Works best with lightly fixed tissues, or Bouin's fixed material, although there are reports of traditional formalin fixation also working with antigen recovery. Positive control: Brain. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS buffer, pH 7.2, 0.09 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- avoid freeze thaw cycles. Store undiluted.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.
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- PLP1 (Proteolipid Protein 1 (PLP1))
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别名
- PLP1 / Myelin PLP
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背景
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Name/Gene ID: PLP1
Synonyms: PLP1, HLD1, PMD, Myelin proteolipid protein, Proteolipid protein 1, SPG2, Lipophilin, MMPL, PLP, PLP/DM20 -
基因ID
- 5354
抗原
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