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PDGF-AA Homodimer 抗体

适用: 人 WB, IHC, ELISA, IHC (p), Neut 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN1103534
发货至: 中国
  • 抗原
    PDGF-AA Homodimer
    适用
    • 27
    • 6
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    宿主
    • 22
    • 6
    • 1
    • 1
    克隆类型
    • 24
    • 6
    多克隆
    标记
    • 17
    • 6
    • 4
    • 1
    • 1
    • 1
    非结合性
    应用范围
    • 30
    • 19
    • 11
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut)
    品牌
    IHC-plus™
    特异性
    Human PDGF-AA
    纯化方法
    Immunoaffinity purified
    免疫原
    E.coli derived recombinant Human PDGF-AA.

    Type of Immunogen: Cell extract
    亚型
    IgG
  • 应用备注
    Approved: ELISA, IHC, IHC-P (5 μg/mL), Neut (5 - 7 μg/mL), WB (0.1 - 0.2 μg/mL)

    Usage: Immunohistochemistry: LS-B7488 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hPDGF-AA (5 ng/mL), a concentration of 0.13-0.20 μg/mL of this antibody is required. ELISA: To detect hPDGF-AA by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human PDGF-AA (LS-C104676) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hPDGF-AA. Western Blot: To detect hPDGF-AA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-AA is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Sterile water
    浓度
    Lot specific
    缓冲液
    Lyophilized from PBS,  pH 7.2
    注意事项
    Avoid freeze/thaw cycles.
    储存条件
    4 °C,-20 °C
    储存方法
    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • 抗原
    PDGF-AA Homodimer
    别名
    PDGF-AA
    别名
    PDGF-A antibody, PDGF1 antibody, AA409063 antibody, CD142 antibody, Cf-3 antibody, Cf3 antibody, TF antibody, PDGFACP antibody, platelet derived growth factor subunit A antibody, coagulation factor III antibody, platelet derived growth factor, alpha antibody, PDGFA antibody, F3 antibody, Pdgfa antibody
    背景
    Name/Gene ID: PDGFA
    Family: PDGF

    Synonyms: PDGFA, PDGF-A, PDGF A-chain, PDGF subunit A, PDGF-1, PDGF1
    基因ID
    5154
    UniProt
    P04085
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