PDGF-AA Homodimer 抗体
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北京 101111
Quick Overview for PDGF-AA Homodimer 抗体 (ABIN1103534)
抗原
适用
宿主
克隆类型
标记
应用范围
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特异性
- Human PDGF-AA
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纯化方法
- Immunoaffinity purified
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免疫原
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E.coli derived recombinant Human PDGF-AA.
Type of Immunogen: Cell extract -
亚型
- IgG
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anti-PDGF-AA Homodimer (AA 87-211) antibody
适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-PDGF-AA Homodimer (AA 87-211) antibody适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C1 unconjugated
anti-PDGF-AA Homodimer (AA 94-194) antibody适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-PDGF-AA Homodimer (AA 90-190) antibody适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: ELISA, IHC, IHC-P (5 μg/mL), Neut (5 - 7 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Immunohistochemistry: LS-B7488 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hPDGF-AA (5 ng/mL), a concentration of 0.13-0.20 μg/mL of this antibody is required. ELISA: To detect hPDGF-AA by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human PDGF-AA (LS-C104676) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hPDGF-AA. Western Blot: To detect hPDGF-AA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-AA is 1.5-3 ng/lane, under either reducing or non-reducing conditions. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Sterile water
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS, pH 7.2
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注意事项
- Avoid freeze/thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- PDGF-AA Homodimer
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别名
- PDGF-AA
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背景
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Name/Gene ID: PDGFA
Family: PDGF
Synonyms: PDGFA, PDGF-A, PDGF A-chain, PDGF subunit A, PDGF-1, PDGF1 -
基因ID
- 5154
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UniProt
- P04085
抗原
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