Myc Tag 抗体 (AA 410-419)
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Quick Overview for Myc Tag 抗体 (AA 410-419) (ABIN1103152)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 410-419
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特异性
- This affinity purified antibody is directed against human c-Myc and is useful in determining its presence in various assays. This polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
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纯化方法
- Affinity purified
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过滤
- Sterile filtered
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免疫原
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This antibody was purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide, E-Q-K-L-I-S-E-E-D-L, conjugated to KLH using maleimide. The sequence corresponds to amino acids 410-419 of human c-Myc.
Type of Immunogen: Purified protein -
亚型
- IgG
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anti-Myc Tag (AA 408-439) antibody
Animal-Free 适用: 标记 WB, ELISA, IF, FACS 宿主: 兔 Monoclonal TUN219-2C1 (9E10) unconjugated Recombinant Antibody
anti-Myc Tag (AA 408-439) antibodyAnimal-Free 适用: 标记 WB, ELISA, IF, FACS 宿主: 小鼠 Monoclonal TUN219-2C1 (9E10) unconjugated Recombinant Antibody
anti-Myc Tag (AA 408-439) antibodyAnimal-Free 适用: 标记 WB, ELISA, IF, FACS 宿主: Human Monoclonal TUN219-2C1 (9E10) unconjugated Recombinant Antibody
anti-Myc Tag (C-Term) antibody适用: 人 WB, IP, ELISA, IHC (fro), IHC (p), FACS, ICC, ICFC 宿主: 小鼠 Monoclonal 9E10 unconjugated
anti-Myc Tag (C-Term) antibody (Biotin)适用: 人 WB, IP, ELISA, IHC (fro), IHC (p), FACS, ICC, ICFC 宿主: 小鼠 Monoclonal 9E10 Biotin
anti-Myc Tag (C-Term) antibody (FITC)适用: 人 FACS, ICFC 宿主: 小鼠 Monoclonal 9E10 FITC
anti-Myc Tag antibodyWB, IP, ELISA, IF 宿主: 小鼠 Monoclonal 2G8D5 unconjugated
anti-Myc Tag (AA 408-439) antibodyWB, ELISA 宿主: 小鼠 Monoclonal 9E10- unconjugated
anti-Myc Tag (AA 410-419) antibodyWB 宿主: 山羊 Polyclonal unconjugated
anti-Myc Tag (AA 423-435) antibodyWB, IP, IHC (p), IHC, FACS 宿主: 小鼠 Monoclonal 9E10 unconjugated
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应用备注
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Approved: ELISA, IHC, WB (1:500 - 1:5000)
Usage: Anti-Myc has utility to detect the fusion protein of the Myc epitope cloned along with the target gene. As such, anti-Myc/Myc can be used to identify fusion proteins containing the Myc epitope. The antibody recognizes the Myc tag fused either to the AMINO- or CARBOXY- termini of targeted proteins. This antibody is suitable for ELISA and western blotting and was tested against both the immunizing peptide and Myc-tagged recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide, sterile filtered
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- avoid freeze thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- Myc Tag
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物质类
- Tag
抗原
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