LINE-1 抗体
The 小鸡 多克隆 anti-LINE-1 antibody (ABIN1102848) specifically detects LINE-1 in ELISA 和 WB.
The antibody is reactive with 人 samples.
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产品编号 ABIN1102848
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for LINE-1 抗体 (ABIN1102848)
抗原
LINE-1
适用
人
宿主
小鸡
克隆类型
多克隆
应用范围
ELISA, Western Blotting (WB)
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特异性
- Reactivity occurs against human L1/ORF2 protein and is useful in determining its presence in various assays. A BLAST analysis was used to suggest cross reactivity with L1/ORF2 proteins from chimpanzee sources based on 100 % homology with the immunizing sequences, and from macaque, fruit fly, cattle, dog, opossum, and rat sources based on 69 - 88 % homology with the immunizing sequences.
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纯化方法
- Protein A purified
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过滤
- Sterile filtered
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免疫原
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This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with two synthetic peptides conjugated to keyhole limpet hemocyanin (KLH). The peptides correspond to regions within the endonuclease domain of L1/ORF2 protein.
Type of Immunogen: Synthetic peptide -
亚型
- IgY
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应用备注
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Approved: ELISA, WB (1:2000 - 1:10000)
Usage: This IgY fraction antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 149 kD in size corresponding to L1 protein by western blotting in the appropriate cell lysate or extract. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide, sterile filtered
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- avoid freeze thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- LINE-1
抗原
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