GM-CSF 抗体
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北京 101111
Quick Overview for GM-CSF 抗体 (ABIN1100979)
抗原
See all GM-CSF (CSF2) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This purified antibody has been heated to 56 °C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human GM-CSF in cell supernatants and certain body fluids. This antibody is useful for neutralization of human GM-CSF in bioassays. For neutralization, it is recommended to incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
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纯化方法
- Purified, heated to 56°C for 30 minutes
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免疫原
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This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human GM-CSF protein.
Type of Immunogen: Recombinant protein -
亚型
- IgG
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应用备注
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Approved: ELISA, WB (1:500 - 1:2000)
Usage: This purified antibody has been tested for use in neutralizations, ELISA and western blotting. Reactivity is also expected in radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/ul by the LAL method. By western blot a band approximately 14.5 kD in size corresponding to human GM-CSF protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- deionized water
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浓度
- Lot specific
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缓冲液
- 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2
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注意事项
- avoid freeze thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
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别名
- CSF2 / GMCSF / GM-CSF
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背景
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Name/Gene ID: CSF2
Synonyms: CSF2, Colony-stimulating factor, CSF, GM-CSF, Sargramostim, Molgramostin, GMCSF -
基因ID
- 1437
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UniProt
- P04141
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途径
- JAK/STAT Signaling, Cellular Response to Molecule of Bacterial Origin
抗原
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