Malic Dehydrogenase (MDH) 抗体
适用: Pig
IP
宿主: 兔
Polyclonal
unconjugated
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- 抗原
- Malic Dehydrogenase (MDH)
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适用
- Pig
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- 非结合性
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应用范围
- Immunoprecipitation (IP)
- 特异性
- Cross-reactivities against enzymes of other sources may occur but have not been determined.
- 产品特性
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The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
Physicochemical characteristics: PAb concentration 1 mg/0.5ml. No foreign proteins added. - 纯化方法
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Antisera
Specific polyclonal antibody fractions were prepared by solid phase affinity chromatography. - 免疫原
- Malic dehydrogenase isolated and purified from porcine heart. Freund's complete adjuvant is used in the first step of the immunization procedure.
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- 应用备注
- Working dilutions in non-precipitating antibody-binding techniques may vary widely, but may be between 1/10,000 and 1/100,000.
- 说明
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Physical form: Purified hyperimmune rabbit IgG antibodies lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2), stabilized with dextran.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- Phosphate buffered saline (PBS, pH 7.2). No foreign protein added.
- 储存液
- Without preservative
- 注意事项
- The lyophilised PAb fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 0.5 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
- 储存条件
- 4 °C
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- 抗原
- Malic Dehydrogenase (MDH)
- 别名
- MDH antibody, Mdh-2 antibody, Mor-1 antibody, Mor1 antibody, MMDH antibody, malate dehydrogenase 2, NAD (mitochondrial) antibody, malate dehydrogenase 2 antibody, Mdh2 antibody, MDH2 antibody
- 背景
- This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer's own choice.
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