Serum 抗体
This 山羊 多克隆 antibody specifically detects Serum in IEP 和 ODD. It exhibits reactivity toward Pig.
产品编号 ABIN1099690
发货至:
中国
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Contact Info
Our Local Distributor
中国
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北京 101111
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Beijing Economic Technological Development Area
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Quick Overview for Serum 抗体 (ABIN1099690)
抗原
Serum
适用
Pig
宿主
山羊
克隆类型
多克隆
标记
非结合性
应用范围
Immunoelectrophoresis (IEP), Ouchterlony Double Diffusion (ODD)
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原理
- Goat anti Swine Serum proteins
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特异性
- Precipitating polyclonal Goat antiSerum to Swine Serum proteins
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交叉反应 （详细）
- Inter-species cross reactivity is a normal feature of antibodies to animal proteins since homologous proteins of different species frequently share antigenic determinants. This antiSerum has not been adsorbed for such cross reactivity. Consequently it is not species-specific. In immunoelectrophoresis anti Bovine and Human Serum cross-reactions has been observed.
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产品特性
- In immunoelectrophoresis against pooled serum and enriched serum proteins fractions precipitation can be observed of not less than 12 individual proteins components. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion (Ouchterlony) to identify the serum protein pattern, or the presence or absence of an individual component. To evaluate the purity of an isolated serum protein including immunoglobulins. Since immunoprecipitation depends on a correct antigen/antibody concentration ratio (zone of equivalence) in the gel medium, the protein analysis by immunoelectrophoresis of serum or any other biological fluid or protein fraction should include different proportions of the reactants. It is not possible to obtain an optimal protein pattern in a single analysis. The electroendosmosis effect of different types of agar on proteins with a different net charge can be used to optimize the resolution power of the test system. Agar Nordic Nr. 2 contains sufficient positively charged ions to optimize the resolution of the proteins in the beta-gamma regions, while the alpha regions will become more dense. Highly purified agar (Agar Nordic nr. 1) with low electroendosmosis favours the resolution of the proteins in the alpha regions, while the major components in the beta-gamma region can still be identified.
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纯化方法
- Delipidated, heat inactivated, lyophilized, stable whole antiserum
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免疫原
- Pooled whole swine serum and partly purified serum fractions. Freund's complete adjuvant is used in the first step of the immunization procedure.
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应用备注
- Precipitation assays. In immunoelectrophoresis use 2 μL serum or equivalent against 120 μL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μL antiserum in 3 mm diameter center well and 2 μL serum samples (neat and serially diluted in 2 mm diameter peripheral wells.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.
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缓冲液
- Delipidated, heat inactivated, lyophilized, stable whole antiserum. 0.1 % Sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.
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- Serum
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别名
- Serum Proteins
抗原
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