VGlut3 Transporter (AA 546-588), (C-Term) 抗体
The 小鼠 单克隆 anti-VGlut3 Transporter antibody (Clone S34-34) (ABIN1098207) specifically detects VGlut3 Transporter in IHC, WB 和 IF/ICC.
The antibody is reactive with 大鼠 samples.
产品编号 ABIN1098207
发货至:
中国
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Quick Overview for VGlut3 Transporter (AA 546-588), (C-Term) 抗体 (ABIN1098207)
抗原
VGlut3 Transporter
适用
大鼠
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (fixed cells) (IF/ICC)
克隆位点
S34-34
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抗原表位
- AA 546-588, C-Term
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原理
- Anti-VGlut3 Transporter Mouse Monoclonal Antibody
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特异性
- This antibody recognizes human, mouse, and rat VGlut3. It does not cross-react with VGlut1 or VGlut2.
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交叉反应
- 人, 小鼠, 大鼠
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交叉反应 （详细）
- No cross Reactivity with Vglut1 and Vglut2
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纯化方法
- Purified by Protein G affinity chromatography.
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免疫原
- Fusion protein corresponding to aa 546-588 (cytoplasmic C-terminus) of rat VGlut3 (accession no. NP_74947.1).
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亚型
- IgG1
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应用备注
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Immunoblotting: use at 1-2 μg/mL. A band of ~65 kDa is detected.
Immunohistochemistry: use at 1-5 μg/mL.
These are recommended concentrations.
Enduser should determine optimal concentrations for their applications.
Positive control: CV-1 fibroblasts transfected with VGlut3. -
限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Dilute in PBS or medium that is identical to that used in the assay system.
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.
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- VGlut3 Transporter
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背景
- Vesicular glutamate transporter 3,Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate. {PubMed:12097496, PubMed:12388773}.,The ATP-dependent, chloride-sensitive vesicular glutamate transporters include VGlut1, VGlut2, and VGlut3. VGlut3 is a multipass membrane protein restricted to synaptic vesicles of glutamatergic neurons. It is specifically expressed in amygdala, cerebellum, hippocampus, medulla, spinal cord, and thalamus.,Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Membrane, Cell junction, synapse, synaptosome,VGluT3, Solute carrier family 17 member 8
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UniProt
- Q7TSF2
抗原
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