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ACLY 抗体 (AA 306-502)

Name: ACLY 抗体 (AA 306-502)
Brand: antibodies-online
SKU: ABIN1098143
Availability: OutOfStock

This anti-ACLY antibody is a 小鼠单克隆 antibody detecting ACLY in WB, ELISA, IHC, FACS 和 ICC. Suitable for 人, 小鼠, 大鼠和猴. This Primary Antibody has been cited in 2+ publications.

Western blot analysis using ACLY mAb against human ACLY recombinant protein. (ACLY 抗体 (AA 306-502))

Flow cytometric analysis of HeLa cells using ACLY mouse mAb (green) and negative control (purple). (ACLY 抗体 (AA 306-502))

FACS

Immunofluorescence analysis of HeLa cells using ACLY mouse mAb (green). (ACLY 抗体 (AA 306-502))

Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using ACLY mouse mAb with DAB staining. (ACLY 抗体 (AA 306-502))

IHC

Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using ACLY mouse mAb with DAB staining. (ACLY 抗体 (AA 306-502))

IHC

Western blot analysis using ACLY mouse mAb against HeLa (1), NIH3T3 (2), C6 (3), COS7 (4), and Raji (5) cell lysate. (ACLY 抗体 (AA 306-502))

Black line: Control Antigen (100 ng), Purple line: Antigen(10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng), (ACLY 抗体 (AA 306-502))

ELISA

7 images

(2 references)

产品编号 ABIN1098143

发货至: 中国

Quick Overview for ACLY 抗体 (AA 306-502) (ABIN1098143)

抗原

See all ACLY 抗体

ACLY (ATP Citrate Lyase (ACLY))

适用

人, 小鼠, 大鼠, 猴

宿主

小鼠

克隆类型

单克隆

标记

This ACLY antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)

克隆位点

5F8D11

抗原表位
19

15

7

6

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 306-502

原理

ACLY Antibody

纯化方法

Purified antibody

免疫原

Purified recombinant fragment of human ACLY (AA: 306-502 ) expressed in E. Coli.

亚型

IgG1
anti-ATP Citrate Lyase (ACLY) (AA 752-1101) antibody
ACLY 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-ATP Citrate Lyase (ACLY) (pSer455) antibody
ACLY 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated

anti-ATP Citrate Lyase (ACLY) (AA 306-502) antibody
ACLY 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, IHC, FACS, IF/ICC 宿主: 小鼠 Monoclonal unconjugated

anti-ATP Citrate Lyase (ACLY) (AA 4-265) antibody
ACLY 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-ATP Citrate Lyase (ACLY) antibody
ACLY 适用: 人 WB, ELISA, IF, FACS 宿主: 兔 Monoclonal 3A5 unconjugated Recombinant Antibody

anti-ATP Citrate Lyase (ACLY) (C-Term) antibody
ACLY 适用: 人, 小鼠 WB, FACS, ICC 宿主: 小鼠 Monoclonal unconjugated

anti-ATP Citrate Lyase (ACLY) (Middle Region) antibody
ACLY 适用: 人, 小鼠, 大鼠, Pig, Cow, 兔, 斑马鱼, 绵羊, 犬, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-ATP Citrate Lyase (ACLY) antibody
ACLY 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-ATP Citrate Lyase (ACLY) (Ser1227) antibody
ACLY 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-ATP Citrate Lyase (ACLY) (AA 793-1008) antibody
ACLY 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

ELISA: 1/10000
FCM: 1/200 - 1/400
ICC: 1/50

限制

仅限研究用
状态

Liquid

缓冲液

Purified antibody in PBS with 0.05 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Chu, Lin, Hendel, Kulpa, Brownsey, Johnson: "ATP-citrate lyase reduction mediates palmitate-induced apoptosis in pancreatic beta cells." in: The Journal of biological chemistry, Vol. 285, Issue 42, pp. 32606-15, (2010) (PubMed).

Beckner, Fellows-Mayle, Zhang, Agostino, Kant, Day, Pollack: "Identification of ATP citrate lyase as a positive regulator of glycolytic function in glioblastomas." in: International journal of cancer. Journal international du cancer, Vol. 126, Issue 10, pp. 2282-95, (2010) (PubMed).
抗原

ACLY (ATP Citrate Lyase (ACLY))

别名

ACLY

背景

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene.

分子量

125 kDa

基因ID

47

UniProt

P53396

途径

Warburg Effect