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PLK1 抗体 (AA 331-508)

Name: PLK1 抗体 (AA 331-508)
Brand: antibodies-online
SKU: ABIN1098105
Availability: OutOfStock

This anti-PLK1 antibody is a 小鼠单克隆 antibody detecting PLK1 in WB, ELISA, IHC 和 FACS. Suitable for 人和小鼠. This Primary Antibody has been cited in 3+ publications.

Western blot analysis using PLK1 mouse mAb against K562 (1) and Raji (2) cell lysate. (PLK1 抗体 (AA 331-508))

Black line: Control Antigen (100 ng), Purple line: Antigen(10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng), (PLK1 抗体 (AA 331-508))

ELISA

Flow cytometric analysis of NIH3T3 cells using PLK1 mouse mAb (green) and negative control (red). (PLK1 抗体 (AA 331-508))

FACS

Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using PLK1 mouse mAb with DAB staining. (PLK1 抗体 (AA 331-508))

IHC

Western blot analysis using PLK1 mAb against human PLK1 recombinant protein. (PLK1 抗体 (AA 331-508))

Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PLK1 mouse mAb with DAB staining. (PLK1 抗体 (AA 331-508))

IHC

6 images

(3 references)

产品编号 ABIN1098105

发货至: 中国

Datasheet as PDF

Quick Overview for PLK1 抗体 (AA 331-508) (ABIN1098105)

抗原

See all PLK1 抗体

PLK1 (Polo-Like Kinase 1 (PLK1))

适用

人, 小鼠

宿主

小鼠

克隆类型

单克隆

标记

This PLK1 antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

克隆位点

3C11

抗原表位
22

18

15

12

8

5

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 331-508

原理

PLK1 Antibody

纯化方法

Purified antibody

免疫原

Purified recombinant fragment of human PLK1 (AA: 331-508) expressed in E. Coli.

亚型

IgG1
anti-Polo-Like Kinase 1 (PLK1) (AA 1-603) antibody
PLK1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 4G11 unconjugated

anti-Polo-Like Kinase 1 (PLK1) (AA 1-603) antibody
PLK1 适用: 人 WB, ELISA, IF, IP, PLA 宿主: 小鼠 Monoclonal 2G12 unconjugated

anti-Polo-Like Kinase 1 (PLK1) antibody
PLK1 适用: 人 WB, IHC, IP, IHC (p), FACS 宿主: 小鼠 Monoclonal 5C1 unconjugated

anti-Polo-Like Kinase 1 (PLK1) antibody
PLK1 适用: 人, 犬 WB, IHC, IP, IHC (p) 宿主: 小鼠 Monoclonal 6C6 unconjugated

anti-Polo-Like Kinase 1 (PLK1) antibody
PLK1 适用: 人 WB, IHC, IP, IHC (p) 宿主: 小鼠 Monoclonal 1D4 unconjugated

anti-Polo-Like Kinase 1 (PLK1) antibody
PLK1 适用: 人, 犬, 猴 WB, IF, IP, FACS 宿主: 小鼠 Monoclonal 8C12 unconjugated

anti-Polo-Like Kinase 1 (PLK1) (AA 201-300) antibody
PLK1 适用: 人 WB, ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Polo-Like Kinase 1 (PLK1) antibody
PLK1 适用: 人 WB, IHC, IP, IHC (p), FACS 宿主: 小鼠 Monoclonal 3F12 unconjugated

anti-Polo-Like Kinase 1 (PLK1) (AA 331-508) antibody
PLK1 适用: 人 ELISA, ICC 宿主: 小鼠 Monoclonal 1D1 unconjugated

anti-Polo-Like Kinase 1 (PLK1) (AA 331-508) antibody
PLK1 适用: 人 WB, ELISA, IF/ICC 宿主: 小鼠 Monoclonal unconjugated

应用备注

ELISA: 1/10000
FCM: 1/200 - 1/400

限制

仅限研究用
状态

Liquid

缓冲液

Purified antibody in PBS with 0.05 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Lin, Sun, Wang: "Suppression of Polo like kinase 1 (PLK1) by p21(Waf1) mediates the p53-dependent prevention of caspase-independent mitotic death." in: Cellular signalling, Vol. 23, Issue 11, pp. 1816-23, (2011) (PubMed).

Yoshioka, Kimura, Saio, Era, Okano: "Plk1 is negatively regulated by RNF8." in: Biochemical and biophysical research communications, Vol. 410, Issue 1, pp. 57-61, (2011) (PubMed).

Susilowati, Okamura, Hirota, Shono, Yoshida, Murakami, Tabata, Nagamune, Haneji, Miyake: "Intermedilysin induces EGR-1 expression through calcineurin/NFAT pathway in human cholangiocellular carcinoma cells." in: Biochemical and biophysical research communications, Vol. 404, Issue 1, pp. 57-61, (2011) (PubMed).
抗原

PLK1 (Polo-Like Kinase 1 (PLK1))

别名

PLK1

背景

Polo-like kinase 1 (Plk1) plays key roles in many aspects of mitosis. Suppression of Plk1 by p21(Waf1) is responsible for p53-dependent protection against adriamycin-induced caspase-independent mitotic death.

分子量

68 kDa

基因ID

5347

UniProt

P53350

途径

Cell Division Cycle, M Phase