CENPE 抗体
(10 references)Quick Overview for CENPE 抗体 (ABIN108562)
抗原
See all CENPE 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Affinity purified by ion exchange chromatography
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免疫原
- Recombinant full length CENPE protein (human)
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亚型
- IgG1 kappa
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anti-Centromere Protein E, 312kDa (CENPE) antibody
CENPE 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein E, 312kDa (CENPE) antibodyCENPE 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein E, 312kDa (CENPE) (AA 2450-2590) antibodyCENPE 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein E, 312kDa (CENPE) (C-Term) antibodyCENPE 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein E, 312kDa (CENPE) (C-Term) antibodyCENPE 适用: 人 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein E, 312kDa (CENPE) antibodyCENPE 适用: 人 WB, IP, ICC, IF 宿主: 小鼠 Monoclonal 1H12 unconjugated
anti-Centromere Protein E, 312kDa (CENPE) (AA 2542-2701) antibodyCENPE 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein E, 312kDa (CENPE) (AA 2222-2361) antibodyCENPE 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal antibody dilution should be determined by titration, however as a guideline try IP: Use at μg/mg of lysate. Use at a concentration of 3 μg/mL per 300 μg of lysate WB: Use a concentration of 0.5 - 1 μg/mL. Predicted molecular weight: 312 kDa. Only suitable for WB if IP is performed first Flow CytFlow Cyt: Use 1 μg for 106 cells
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说明
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Myeloma, fusion partners: SP2/0
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Purified antibody (from supernatant) containing PBS 0.1 % sodium azide,
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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: "Midbody assembly and its regulation during cytokinesis." in: Molecular biology of the cell, Vol. 23, Issue 6, pp. 1024-34, (2012) (PubMed).
: "Small GTPase Rab5 participates in chromosome congression and regulates localization of the centromere-associated protein CENP-F to kinetochores." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 42, pp. 17337-42, (2011) (PubMed).
: "Aurora kinases and protein phosphatase 1 mediate chromosome congression through regulation of CENP-E." in: Cell, Vol. 142, Issue 3, pp. 444-55, (2010) (PubMed).
: "Telomere disruption results in non-random formation of de novo dicentric chromosomes involving acrocentric human chromosomes." in: PLoS genetics, Vol. 6, Issue 8, (2010) (PubMed).
: "Mediator of DNA damage checkpoint 1 (MDC1) regulates mitotic progression." in: The Journal of biological chemistry, Vol. 284, Issue 49, pp. 33939-48, (2009) (PubMed).
: "Glycogen synthase kinase 3beta interacts with and phosphorylates the spindle-associated protein astrin." in: The Journal of biological chemistry, Vol. 283, Issue 4, pp. 2454-64, (2008) (PubMed).
: "PIASgamma is required for faithful chromosome segregation in human cells." in: PLoS ONE, Vol. 1, pp. e53, (2007) (PubMed).
: "Farnesyl transferase inhibitors impair chromosomal maintenance in cell lines and human tumors by compromising CENP-E and CENP-F function." in: Molecular cancer therapeutics, Vol. 6, Issue 4, pp. 1317-28, (2007) (PubMed).
: "Tankyrase-1 polymerization of poly(ADP-ribose) is required for spindle structure and function." in: Nature cell biology, Vol. 7, Issue 11, pp. 1133-9, (2005) (PubMed).
: "CENP-E, a novel human centromere-associated protein required for progression from metaphase to anaphase." in: The EMBO journal, Vol. 10, Issue 5, pp. 1245-54, (1991) (PubMed).
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: "Midbody assembly and its regulation during cytokinesis." in: Molecular biology of the cell, Vol. 23, Issue 6, pp. 1024-34, (2012) (PubMed).
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- CENPE (Centromere Protein E, 312kDa (CENPE))
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别名
- CENPE
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背景
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Synonyms:
CENP E antibody,Centromere autoantigen E antibody,Centromere protein E 312kDa antibody,Centromereprotein E antibody,Centromeric protein E antibody,KIF10 antibody,Kinesin family member 10 antibody,Kinesinrelated protein antibody -
基因ID
- 1062, 296137
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OMIM
- 117143
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UniProt
- O08901
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途径
- M Phase, Maintenance of Protein Location
抗原
-
