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A2BP1 抗体 (AA 394-646)

A2BP1 适用: Chlamydomonas reinhardtii WB, IF 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN108467
发货至: 中国
  • 抗原 See all A2BP1 抗体
    A2BP1 (Ataxin 2-Binding Protein 1 (A2BP1))
    抗原表位
    • 15
    • 8
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 394-646
    适用
    • 38
    • 17
    • 9
    • 8
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Chlamydomonas reinhardtii
    宿主
    • 33
    • 6
    克隆类型
    • 33
    • 6
    多克隆
    标记
    • 18
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This A2BP1 antibody is un-conjugated
    应用范围
    • 31
    • 13
    • 13
    • 7
    • 6
    • 6
    • 3
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunofluorescence (IF)
    交叉反应 (详细)
    Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
    预测反应
    Chlamydomonas reinhardtii
    产品特性
    Expected / apparent Molecular Weight of the Antigene: 122 / 120 kDa
    纯化方法
    serum
    免疫原
    residues 394-646 from Chlamydomonas reinhardtii FOX1 A8IZT9 fused to TrxA
    Top Product
    Discover our top product A2BP1 Primary Antibody
  • 应用备注
    1: 1000 - 1: 5000 with standard ECL (WB),
    说明

    This antibody has been used on Chlamydomonas cells expressing endogenous Fox1 and on HEK293 cells transiently expressing Fox1. Cells were resuspended in PBS plated on poly-lysine coated coverslips, fixed in 3.7% formaldehyde. Blocked in 3% BSA in PBS. 1:500 dilution of anti-FOX1 in 3% BSA. Secondary antibody was anti-rabbit Alexa fluor 488 1:300.

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    For reconstitution add 200 μL of sterile water.
    注意事项
    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
    储存条件
    -20 °C
    储存方法
    store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Terauchi, Peers, Kobayashi, Niyogi, Merchant: "Trophic status of Chlamydomonas reinhardtii influences the impact of iron deficiency on photosynthesis." in: Photosynthesis research, Vol. 105, Issue 1, pp. 39-49, (2010) (PubMed).

  • 抗原
    A2BP1 (Ataxin 2-Binding Protein 1 (A2BP1))
    别名
    FOX1 (A2BP1 产品)
    别名
    A2BP1 antibody, fox1 antibody, a2bp1 antibody, zgc:103635 antibody, 2BP1 antibody, FOX-1 antibody, FOX1 antibody, HRNBP1 antibody, A2bp antibody, A2bp1 antibody, Hrnbp1 antibody, fox-1 antibody, RNA binding protein, fox-1 homolog 1 antibody, RNA binding fox-1 homolog 1 antibody, multicopper ferroxidase antibody, RNA binding protein, fox-1 homolog (C. elegans) 1 antibody, RBFOX1 antibody, rbfox1 antibody, FOX1 antibody, Rbfox1 antibody
    背景
    A copper-containing protein in Chlamydomonas reinhardtii is a plasma membrane ferroxidase, encoded by the FOX1 gene, involved in Fe assimilation. FOX1 is most highly related to the mammalian homologues hephaestin and ceruloplasmin, its occurrence and pattern of expression in Chlamydomonas indicate a role for copper in iron assimilation in a photosynthetic species.
    分子量
    expected: 122 kDa, apparent: 120 kDa
    基因ID
    394646
    UniProt
    A8IZT9
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