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A2BP1 抗体 (AA 394-646)

There is 1 publication for this product available. The 兔 多克隆 anti-A2BP1 antibody is suitable to detect A2BP1 in samples from Chlamydomonas reinhardtii. It has been validated for WB 和 IF.
产品编号 ABIN108467
发货至: 中国
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Quick Overview for A2BP1 抗体 (AA 394-646) (ABIN108467)

抗原

See all A2BP1 抗体
A2BP1 (Ataxin 2-Binding Protein 1 (A2BP1))

适用

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Chlamydomonas reinhardtii

宿主

  • 36
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克隆类型

  • 36
  • 5
多克隆

标记

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This A2BP1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF)
  • 抗原表位

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    AA 394-646

    交叉反应 (详细)

    Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

    预测反应

    Chlamydomonas reinhardtii

    产品特性

    Expected / apparent Molecular Weight of the Antigene: 122 / 120 kDa

    纯化方法

    serum

    免疫原

    residues 394-646 from Chlamydomonas reinhardtii FOX1 A8IZT9 fused to TrxA
  • 应用备注

    1: 1000 - 1: 5000 with standard ECL (WB),

    说明

    This antibody has been used on Chlamydomonas cells expressing endogenous Fox1 and on HEK293 cells transiently expressing Fox1. Cells were resuspended in PBS plated on poly-lysine coated coverslips, fixed in 3.7% formaldehyde. Blocked in 3% BSA in PBS. 1:500 dilution of anti-FOX1 in 3% BSA. Secondary antibody was anti-rabbit Alexa fluor 488 1:300.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    For reconstitution add 200 μL of sterile water.

    注意事项

    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

    储存条件

    -20 °C

    储存方法

    store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Terauchi, Peers, Kobayashi, Niyogi, Merchant: "Trophic status of Chlamydomonas reinhardtii influences the impact of iron deficiency on photosynthesis." in: Photosynthesis research, Vol. 105, Issue 1, pp. 39-49, (2010) (PubMed).

  • 抗原

    A2BP1 (Ataxin 2-Binding Protein 1 (A2BP1))

    别名

    FOX1

    背景

    A copper-containing protein in Chlamydomonas reinhardtii is a plasma membrane ferroxidase, encoded by the FOX1 gene, involved in Fe assimilation. FOX1 is most highly related to the mammalian homologues hephaestin and ceruloplasmin, its occurrence and pattern of expression in Chlamydomonas indicate a role for copper in iron assimilation in a photosynthetic species.

    分子量

    expected: 122 kDa, apparent: 120 kDa

    基因ID

    394646

    UniProt

    A8IZT9
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