A2BP1 抗体 (AA 394-646)
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Quick Overview for A2BP1 抗体 (AA 394-646) (ABIN108467)
抗原
See all A2BP1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 394-646
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原理
- Anti-FOX1 | plasma membrane ferroxidase FLP1
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交叉反应 (详细)
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Chlamydomonas reinhardtii
Not reactive in: No confirmed exceptions from predicted reactivity are currently known -
预测反应
- Chlamydomonas reinhardtii
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纯化方法
- Serum
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免疫原
- residues 394-646 from Chlamydomonas reinhardtii FOX1 A8IZT9 fused to TrxA
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应用备注
- 1 : 1000-1 : 5000 (WB)
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说明
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This antibody has been used on Chlamydomonas cells expressing endogenous Fox1 and on HEK293 cells transiently expressing Fox1. Cells were resuspended in PBS plated on poly-lysine coated coverslips, fixed in 3.7% formaldehyde. Blocked in 3% BSA in PBS. 1:500 dilution of anti-FOX1 in 3% BSA. Secondary antibody was anti-rabbit Alexa fluor 488 1:300.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 200 μL of sterile water
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缓冲液
- Serum
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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: "Trophic status of Chlamydomonas reinhardtii influences the impact of iron deficiency on photosynthesis." in: Photosynthesis research, Vol. 105, Issue 1, pp. 39-49, (2010) (PubMed).
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: "Trophic status of Chlamydomonas reinhardtii influences the impact of iron deficiency on photosynthesis." in: Photosynthesis research, Vol. 105, Issue 1, pp. 39-49, (2010) (PubMed).
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- A2BP1 (Ataxin 2-Binding Protein 1 (A2BP1))
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别名
- FOX1
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背景
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Background: A copper-containing protein in Chlamydomonas reinhardtii is a plasma membrane ferroxidase, encoded by the FOX1 gene, involved in Fe assimilation. FOX1 is most highly related to the mammalian homologues hephaestin and ceruloplasmin, its occurrence and pattern of expression in Chlamydomonas indicate a role for copper in iron assimilation in a photosynthetic species.
Additional information: FOX1 is 10-20-fold induced in iron-deficiency and its occurrence therefore serves as a marker of iron nutrition state in Chlamydomonas. For detection image please refer to the article below.
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分子量
- 122 | 120 kDa
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基因ID
- 394646
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UniProt
- A8IZT9
抗原
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