Uricase (ABC3735) 抗体 (Biotin)
Quick Overview for Uricase (ABC3735) 抗体 (Biotin) (ABIN108081)
抗原
适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-106-092-0100
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Supplier
- Rockland
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原理
- Anti-Uricase Antibody Biotin Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Uricase [Bacillus species].
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产品特性
- Synonyms: goat anti-uricase antibody, biotin conjugated goat anti-uricase antibody, Urate oxidase antibody, Factor-independent urate hydroxylase
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纯化方法
- Anti-Uricase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Uricase [Bacillus species]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Uricase (ABC3735) antibody (HRP)
ABC3735 适用: Bacillus ELISA, IP, WB 宿主: 山羊 Polyclonal HRP
anti-Uricase (ABC3735) antibodyABC3735 适用: Bacillus ELISA, IP, WB 宿主: 山羊 Polyclonal unconjugated
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应用备注
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Application Note: Anti-Uricase Antibody has been tested by ELISA and western blot. This product is suitable to be assayed against 1.0 μg of Uricase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:53,000 of the reconstitution concentration is suggested for Anti-Uricase Antibody.
Western Blot Dilution: 1:500 - 1:1,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:1,000 - 1:2,500
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 10.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Uricase (ABC3735)
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别名
- ABC3735
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物质类
- Dinucleotide
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背景
- Background: Uricase is a peroxisomal enzyme that catalyzes the conversion of urea to allantoin. Uricase performs this action by cleaving the purine ring of uric acid rendering it much more soluble within the body for excretion. Uricase within the Bacillus species is of high importance due to its high activity and thermostability over a wide range of pH 's. Anti-Uricase Antibody is ideal for investigators in Cell Biology and Microbiology research.
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基因ID
- 3202700
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NCBI登录号
- YP_177228
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UniProt
- Q5WBJ3
抗原
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