Trypsinogen 抗体
Quick Overview for Trypsinogen 抗体 (ABIN107877)
抗原
适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-4180-0100
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Supplier
- Rockland
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原理
- Trypsinogen Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Trypsinogen [Bovine Pancreas].
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产品特性
- Synonyms: rabbit anti-Trypsinogen Antibody, Brain trypsinogen antibody, Cationic trypsinogen antibody, Mesotrypsinogen antibody, Pancreatic anionic trypsinogen antibody, Protease, serine, 2 antibody, PRSS2 antibody
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纯化方法
- Anti-Trypsinogen is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Trypsinogen [Bovine Pancreas]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Trypsinogen antibody (Biotin)
适用: Cow WB, ELISA, DB 宿主: 兔 Polyclonal Biotin
anti-Trypsinogen antibody (HRP)适用: Cow WB, ELISA 宿主: 兔 Polyclonal HRP
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应用备注
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Application Note: Trypsinogen Antibody has been tested by ELIDA and dot blot and is assayed against 1.0 μg of Trypsinogen [Bovine Pancreas] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,300 to 1:8,400 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:3,000
ELISA Dilution: 1:5,000 - 1:25,000
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Trypsinogen
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背景
- Background: Trypsinogen, is a precursor to trypsin and a digestive enzyme. It functions as storage of an inactive form of trypsin and kept in the pancreas then released in significant amount, along with other enzymes, when required for protein digestion. Serum trypsinogen is measured using a blood test. Diseases associated with Trypsinogen high levels or mutations are acute pancreatitis and cystic fibrosis. Anti-Trypsinogen Antibody can be useful for researchers interested in studying the serum or pancreas.
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基因ID
- 282603
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UniProt
- Q29463
抗原
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