TYMS 抗体
Quick Overview for TYMS 抗体 (ABIN107488)
抗原
See all TYMS 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-601-199-0100
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Supplier
- Rockland
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原理
- Thymidylate Synthase Antibody
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交叉反应 （详细）
- Because inhibition of Thymidylate Synthase prevents DNA synthesis and cell proliferation, the enzyme is an important target for cancer chemotherapeutic drugs, specifically the fluoropyrimidine group of antineoplastic drugs used to treat solid tumors.
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产品特性
- Synonyms: sheep anti-Thymidylate Synthase Antibody, d TMP synthase antibody, EC 2.1.1.45 antibody, HsT422 antibody, MGC88736 antibody
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纯化方法
- Anti-THYMIDYLATE SYNTHASE IgG fraction is directed against human Thymidylate Synthase and is useful in determining its presence in various assays.
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免疫原
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Immunogen: Anti-THYMIDYLATE SYNTHASE was purified from rabbit serum after repeated immunizations with recombinant human Thymidylate Synthase (36 kDa) produced in E.coli.
Immunogen Type: Recombinant Protein
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亚型
- IgG
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anti-Thymidylate Synthetase (TYMS) (AA 36-313) antibody
TYMS 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Thymidylate Synthetase (TYMS) (AA 111-210) antibodyTYMS 适用: 人 WB, IF, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3A1 unconjugated
anti-Thymidylate Synthetase (TYMS) (AA 60-174) antibodyTYMS 适用: 人 WB, IF, FACS, IHC, ISt, RIA, StM 宿主: 小鼠 Monoclonal TYMS-1884 unconjugated
anti-Thymidylate Synthetase (TYMS) antibodyTYMS 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 1D6 unconjugated
anti-Thymidylate Synthetase (TYMS) antibodyTYMS 适用: 人 WB, IF, IHC (p), IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Thymidylate Synthetase (TYMS) antibodyTYMS 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 5H8 unconjugated
anti-Thymidylate Synthetase (TYMS) (AA 111-210) antibodyTYMS 适用: 人 WB, ELISA, IHC (p), IP 宿主: 小鼠 Monoclonal 2B2 unconjugated
anti-Thymidylate Synthetase (TYMS) (AA 265-294), (C-Term) antibodyTYMS 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB20041 unconjugated
anti-Thymidylate Synthetase (TYMS) (AA 2-54) antibodyTYMS 适用: 人 WB, IF, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-Thymidylate Synthetase (TYMS) (AA 60-174) antibodyTYMS 适用: 人 IF, FACS, ELISA, RIA, Coat 宿主: 小鼠 Monoclonal rTYMS-1884 unconjugated Recombinant Antibody
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应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: THYMIDYLATE SYNTHASE antibody has been tested by western blot. This antibody is suitable for use in ELISA, immunoprecipitation, immunofluorescence microscopy, and immunohistochemistry. The antibody recognizes the expected additional band corresponding to the ternary complex of hTS-dFUMP-reduced folate in HeLa cells treated with the TS inhibitor 5-FUdR. This event occurs in most human breast, colorectal, gastric, head and neck carcinomas. The antibody recognizes the 36,000 MW hTS. Reactivity in other immunoassays is unknown.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:10,000 - 1:50,000
IF Microscopy Dilution: User Optimized
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 10.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- TYMS (Thymidylate Synthetase (TYMS))
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别名
- TYMS
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背景
- Background: Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occurring antisense transcript rTSalpha vary inversely when cell-growth progresses from late-log to plateau phase. Diseases associated with Thymidylate synthase include Rectal Neoplasm and Dihydropyrimidine Dehydrogenase Deficiency. Anti-Thymidylate synthase is useful for researchers interested in Circadian Rhythm, Metabolism and cell cycle research.
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基因ID
- 7298
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UniProt
- P04818
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途径
- Mitotic G1-G1/S Phases
抗原
-
