RNASE1 抗体
Quick Overview for RNASE1 抗体 (ABIN106560)
抗原
See all RNASE1 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-4188-0100
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Supplier
- Rockland
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原理
- Ribonuclease A Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Ribonuclease A [Bovine pancreas].
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产品特性
- Synonyms: rabbit anti-Ribonuclease A Antibody, Pancreatic ribonuclease antibody, Rib 1 antibody, Rib1 antibody, Ribonuclease a family 1 antibody, Ribonuclease pancreatic antibody, Ribonuclease RNase A family 1 antibody, Ribonuclease RNase A family 1 pancreatic antibody
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纯化方法
- Anti-Ribonuclease A is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Ribonuclease A [Bovine pancreas]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1) (AA 26-152) antibody
RNASE1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1) (Center) antibodyRNASE1 适用: 人 WB, ICC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1) (AA 29-156) antibodyRNASE1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1) (AA 31-150) antibodyRNASE1 适用: Cow WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1) antibodyRNASE1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1) (AA 14-42), (N-Term) antibodyRNASE1 适用: 人 WB 宿主: 兔 Polyclonal RB42410 unconjugated
anti-Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1) (AA 26-149) antibodyRNASE1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1) antibodyRNASE1 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1) (C-Term) antibodyRNASE1 适用: 人, Cow, 大鼠, 山羊, 绵羊 WB 宿主: 兔 Polyclonal unconjugated
anti-Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1) (AA 29-156) antibodyRNASE1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: Ribonuclease A Antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Ribonuclease A [Bovine pancreas] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiozoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:40,000 to 1:180,000 is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- RNASE1 (Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1))
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别名
- RNASE1
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背景
- Background: Pancreatic ribonuclease is an endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. It acts on single-stranded and double-stranded RNA. This ribonuclease can destabilize or unwind the DNA helix by complexing with single-stranded DNA, this complex arises by an extended multisite cation-anion interaction between the lysine and arginine residues of the enzyme and the phosphate groups of the nucleotides. It has endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.
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基因ID
- 282340
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UniProt
- P61823
抗原
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