RFX5 抗体 (C-Term)
(1 reference)
Quick Overview for RFX5 抗体 (C-Term) (ABIN106545)
抗原
See all RFX5 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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Supplier Product No.
- 200-401-191
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Supplier
- Rockland
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原理
- RFX5 Antibody
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交叉反应 （详细）
- Anti-RFX5 (C-terminal specific) may react with a minor band at 68 kDa which may represent an RFX5 breakdown product or unmodified RFX5.
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产品特性
- Synonyms: rabbit anti-RFX5 antibody, DNA binding protein RFX 5 antibody, Influences HLA class II expression antibody, Regulatory factor X 5 antibody, Regulatory factor X subunit 5 antibody, Regulatory factor X 5 (influences HLA class II expression) antibody
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纯化方法
- This product was prepared from monospecific antiserum by delipidation, salt fractionation and ion exchange chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: RFX5 (C-terminal specific) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-Regulatory Factor X 5 (RFX5) (AA 1-616) antibody
RFX5 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Regulatory Factor X 5 (RFX5) (AA 367-616) antibodyRFX5 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Regulatory Factor X 5 (RFX5) (Internal Region) antibodyVerified RFX5 适用: 人, 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Regulatory Factor X 5 (RFX5) (AA 1-616) antibodyRFX5 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Regulatory Factor X 5 (RFX5) (AA 516-616) antibodyRFX5 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3B8 unconjugated
anti-Regulatory Factor X 5 (RFX5) antibodyRFX5 适用: 人 WB, ELISA, GS, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Regulatory Factor X 5 (RFX5) antibodyRFX5 适用: 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Regulatory Factor X 5 (RFX5) (N-Term) antibodyRFX5 适用: 人, 犬, 兔, Pig, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Regulatory Factor X 5 (RFX5) (C-Term) antibodyRFX5 适用: 人, 小鼠, 大鼠, 豚鼠, 马, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Regulatory Factor X 5 (RFX5) (N-Term) antibodyRFX5 适用: 人, 犬, 兔 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: This product was assayed by immunoblot and found to be reactive against RFX5 (C-terminal specific) from a variety of fibroblast and B-cell lysates at a dilution of 1:250 to 1:500 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302. Anti-RFX5 (C-terminal specific) is suitable for the detection by immunoblot of human RFX5 a shows a 75 kDa band. This product was also tested in a gel supershift assay and found to be reactive against RFX5 complexes using 0.5 to 1.0 μL per assay.
Western Blot Dilution: 1:500 - 1:3,000
ELISA Dilution: 1:5,000 - 1:25,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Identifying catalytic residues in CPAF, a Chlamydia-secreted protease." in: Archives of biochemistry and biophysics, Vol. 485, Issue 1, pp. 16-23, (2009) (PubMed).
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: "Identifying catalytic residues in CPAF, a Chlamydia-secreted protease." in: Archives of biochemistry and biophysics, Vol. 485, Issue 1, pp. 16-23, (2009) (PubMed).
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- RFX5 (Regulatory Factor X 5 (RFX5))
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别名
- RFX5
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背景
- Background: DNA-binding protein RFX5 activates transcription from class II MHC promoters and recognizes X-boxes. It mediates cooperative binding between RFX and NF-Y. RFX binds the X1 box of MHC-II promoters. RFX5 may be associated with Bare Lymphocyte Syndrome Type li Complementation group C and Mhc Class Ii Deficiency. Anti-RFX5 Antibody is useful for researchers interested in Transcription factors, Tuberculosis, and Allograft rejection.
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基因ID
- 5993
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UniProt
- P48382
抗原
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