PKM 抗体
Quick Overview for PKM 抗体 (ABIN106389)
抗原
See all PKM 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-1178-0100
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Supplier
- Rockland
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原理
- Pyruvate Kinase Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Pyruvate Kinase (Rabbit Muscle).
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产品特性
- Synonyms: goat anti-Pyruvate Kinase Antibody, Pyruvate kinase isozymes M1/M2 antibody, Pyruvate kinase muscle isozyme antibody, Pyruvate kinase 2/3 antibody, Cytosolic thyroid hormone-binding protein antibody, PKM2
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纯化方法
- Anti-Pyruvate Kinase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Pyruvate Kinase (Rabbit Muscle)
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Pyruvate Kinase M1/2 (PKM) (AA 2-531) antibody
PKM 适用: 人 ELISA, WB, IHC, IF, IP, FACS 宿主: 小鼠 Monoclonal 6C3C7 unconjugated
anti-Pyruvate Kinase M1/2 (PKM) (AA 11-221) antibodyPKM 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase M1/2 (PKM) antibodyPKM 适用: 人 ELISA, WB, IHC, IF, IP, FACS 宿主: 兔 Monoclonal 7B2 unconjugated Recombinant Antibody
anti-Pyruvate Kinase M1/2 (PKM) (AA 185-461) antibodyPKM 适用: 人 ELISA, WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase M1/2 (PKM) (AA 11-221) antibodyPKM 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase M1/2 (PKM) (Internal Region) antibodyPKM 适用: 人, 小鼠, 大鼠 ELISA, WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase M1/2 (PKM) (AA 2-192) antibodyPKM 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase M1/2 (PKM) (pSer37) antibodyPKM 适用: 人 ELISA, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase M1/2 (PKM) antibodyPKM 适用: 人, 小鼠, 大鼠 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase M1/2 (PKM) (pSer37) antibodyPKM 适用: 人, 小鼠, 大鼠 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: 1:500 - 1:2,000
Application Note: This affinity purified antibody has been tested in western blot. This antibody is suitable for use in ELISA and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:4,000 - 1:20,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- PKM (Pyruvate Kinase M1/2 (PKM))
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别名
- PKM
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背景
- Background: Pyruvate kinase (PKM) is a glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. There are 4 isozymes of pyruvate kinase in mammals (L, R, M1, M2) encoded by 2 different genes: PKLR and PKM. The L and R isozymes are generated from the PKLR by differential splicing of RNA, the M1 and M2 forms are produced from the PKM gene by differential splicing. L type is major isozyme in the liver, R is found in red cells, M1 is the main form in muscle, heart and brain, and M2 is found in early fetal tissues as well as in most cancer cells. This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate. Anti-Pyruvate Kinase (Rabbit Muscle) Antibody is ideal for investigators in Cancer, Cell Biology, and Neuroscience.
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基因ID
- 100008676
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NCBI登录号
- NP_001182574
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UniProt
- P11974
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途径
- Warburg Effect
抗原
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