Protein G 抗体
Quick Overview for Protein G 抗体 (ABIN106214)
抗原
See all Protein G 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-401-138-0100
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Supplier
- Rockland
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原理
- Protein G Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Protein G.
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产品特性
- Synonyms: rabbit anti-Protein G Antibody, Rabbit anti Protein G, Rabbit anti PG
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纯化方法
- Anti-Protein G is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Protein G [Streptococcus species]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Protein G antibody (Biotin)
适用: Streptococcus ELISA, DB 宿主: 兔 Polyclonal Biotin
anti-Protein G antibody (HRP)适用: Streptococcus ELISA, DB 宿主: 兔 Polyclonal HRP
anti-Protein G antibody (FITC)适用: Streptococcus FACS, IM 宿主: 兔 Polyclonal FITC
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应用备注
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Flow Cytometry Dilution: User Optimized
Immunohistochemistry Dilution: User Optimized
Application Note: Anti-Protein G is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Western Blot Dilution: 1:2,000 - 1:10,000
ELISA Dilution: 1:20,000 - 1:100,000
IF Microscopy Dilution: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Protein G
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背景
- Background: Protein G is a protein that has the property of binging to immunoglobulins. It is a 65- kDa cell surface protein that is commonly used for purifying antibodies through binding to the Fab and Fc regions. Protein G was originally isolated from Streptococcal bacteria. It is similar in properties to Protein A but has unique IgG binding specificities. Native protein G also binds albumin, however Rockland uses recombinant forms of Protein G that only bind to immunoglobulins.
抗原
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