Protein A 抗体
(1 reference)
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Quick Overview for Protein A 抗体 (ABIN106168)
抗原
See all Protein A 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 100-1177
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Supplier
- Rockland
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原理
- Protein A Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Protein A [Staphylococcus aureus].
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产品特性
- Synonyms: Goat anti-Protein A Antibody, Goat anti Protein A
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纯化方法
- This product was prepared from monospecific antiserum by a delipidation and defibrination.
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免疫原
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Immunogen: Protein A [Staphylococcus aureus]
Immunogen Type: Native Protein
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anti-Protein A antibody
适用: Staphylococcus aureus ELISA, WB, IF (p), IF (cc), IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Protein A antibody (Rhodamine)适用: Staphylococcus aureus IF 宿主: 山羊 Polyclonal Rhodamine
anti-Protein A antibody适用: Staphylococcus aureus ELISA, WB, IP 宿主: 山羊 Polyclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: 1:1,000-1:5,000
Application Note: Anti-Protein A has been tested by western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity.
Western Blot Dilution: 1:2,000-1:10,000
ELISA Dilution: 1:20,000-1:100,000
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 90 mg/mL
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缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Defective immuno- and thymoproteasome assembly causes severe immunodeficiency. ..." in: Scientific reports, Vol. 8, Issue 1, pp. 5975, (2018) (PubMed).
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: "Defective immuno- and thymoproteasome assembly causes severe immunodeficiency. ..." in: Scientific reports, Vol. 8, Issue 1, pp. 5975, (2018) (PubMed).
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- Protein A
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背景
- Background: Protein A is 56 kDA surface protein found in the cell wall of Staphylococcus aureus. Protein A has a high affinity to Human IgG, Mouse IgG and others and is therefore suitable for purification and detection of immunoglobulins.
抗原
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