R-Phycoerythrin 抗体 (Texas Red (TR))
Quick Overview for R-Phycoerythrin 抗体 (Texas Red (TR)) (ABIN105639)
抗原
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 600-109-387
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Supplier
- Rockland
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原理
- R-Phycoerythrin Antibody Texas Red™ Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and R-Phycoerythrin conjugated IgG.
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产品特性
- Synonyms: goat anti R-Phycoerythrin Antibody Texas Red™ Conjugated, goat anti Phycoerythrin Antibody Texas Red™ Conjugated
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纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using a R-Phycoerythrin coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with highly purified R-Phycoerythrin from the seaweed gracilaria.
Immunogen Type: Native Protein
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亚型
- IgG
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Labeling Ratio
- 5.9
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anti-Phycoerythrin, R- antibody (Alkaline Phosphatase (AP))
WB, ELISA, IHC 宿主: 山羊 Polyclonal Alkaline Phosphatase (AP)
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应用备注
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Application Note: Anti-R-Phycoerythrin Antibody Texas Red™ Conjugated has been tested by dot blot and western blot. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Suitable for highly specific immunological methods requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.
IF Microscopy Dilution: 1:500 - 1:2,500
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- R-Phycoerythrin (Phycoerythrin, R-)
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别名
- R-Phycoerythrin
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物质类
- Chemical
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背景
- Background: Anti-R-Phycoerythrin Antibody Texas Red™ Conjugated generated in goat recognizes RPE. Phycoerythrin (PE) is a red protein-pigment present in red algae and cryptophytes and an accessory pigment to the main chlorophyll pigments responsible for photosynthesis. R-phycoerythrin is predominantly produced by red algae. The molecular weight of R-PE is 240 kDa. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-R-PE antibody is conjugated to Texas Red™.
抗原
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