Phosphothreonine 抗体
Quick Overview for Phosphothreonine 抗体 (ABIN105601)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 500-301-263
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Supplier
- Rockland
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原理
- Phosphothreonine Antibody
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交叉反应 （详细）
- Reactivity is specific for phosphothreonine and minimal cross reactivity is observed against phosphoserine or phosphotyrosine.
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产品特性
- Synonyms: mouse anti-Phosphothreonine Antibody, mouse anti-pT antibody
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纯化方法
- This product is clarified ascites produced in balb/c mice using clone 18F6.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Phosphothreonine antibody was produced after repeated immunizations of balb/c mice with phosphothreonine conjugated KLH.
Immunogen Type: Other
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亚型
- IgG1 kappa
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anti-Phosphothreonine antibody
WB, IP, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Phosphothreonine antibody (FITC)WB, IP, ELISA, ICC, IF 宿主: 兔 Polyclonal FITC
anti-Phosphothreonine antibody (Biotin)WB, IP, ELISA, ICC, IF 宿主: 兔 Polyclonal Biotin
anti-Phosphothreonine antibody (Atto 488)WB, IP, ELISA, ICC, IF 宿主: 兔 Polyclonal Atto 488
anti-Phosphothreonine antibody (Atto 594)WB, IP, ELISA, ICC, IF 宿主: 兔 Polyclonal Atto 594
anti-Phosphothreonine antibody (Atto 390)WB, IP, ELISA, ICC, IF 宿主: 兔 Polyclonal Atto 390
anti-Phosphothreonine antibody (HRP)WB, IP, ELISA, IHC 宿主: 兔 Polyclonal HRP
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应用备注
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Application Note: This monoclonal antibody has been tested in ELISA and Western Blot and reacts specifically with phosphothreonine and shows minimal reactivity by ELISA, and competitive ELISA, with phosphoserine or phosphotyrosine. The antibody reacts with free phosphothreonine, phosphothreonine conjugated to carriers such as thyroglobulin or BSA, as well as phosphothreonine in proteins of both unstimulated and stimulated cell lysates. Although not tested, this antibody is likely functional in RIA, flow cytometry, immunohistochemistry and immunoprecipitation. Phosphorylation of threonine residues is associated with many growth factors and oncogene protein kinases and is important for cell signaling in activation, proliferation and differentiation.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:4,000 - 1:20,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: None
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Phosphothreonine
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物质类
- Amino Acid
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背景
- Background: Phospho-Threonine Antibody detects proteins and peptides phosphorylated at threonine residues in a manner largely independent of the surrounding amino acid sequence. Phosphorylation of threonine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation.
抗原
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