Beta-Phosphoglucomutase 抗体
Quick Overview for Beta-Phosphoglucomutase 抗体 (ABIN105570)
抗原
适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-101-237-0100
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Supplier
- Rockland
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原理
- Beta-Phosphoglucomutase Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified b-Phosphoglucomutase [Lactococcus lacti].
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产品特性
- Synonyms: goat anti-Beta-Phosphoglucomutase Antibody, goat anti-Beta PGM antibody, Glucose phosphomutase 1 antibody, Glucose phosphomutase antibody, PGM antibody, pgmB antibody
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纯化方法
- Anti-Beta-Phosphoglucomutase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Beta Phosphoglucomutase [Lactococcus lacti]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Phosphoglucomutase, beta- antibody (Biotin)
适用: Lactococcus lacti WB, ELISA 宿主: 山羊 Polyclonal Biotin
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应用备注
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Application Note: Anti-Beta-Phosphoglucomutase Antibody has been tested by ELISA and western blot. This antibody is assayed against 1.0 μg of b-Phosphoglucomutase [Lactococcus lacti] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:60,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:3,000
ELISA Dilution: 1:5,000 - 1:25,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 10.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Beta-Phosphoglucomutase (Phosphoglucomutase, beta-)
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别名
- pgmB
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背景
- Background: Beta-Phosphoglucomutase catalyzes the interconversion of D-glucose 1-phosphate (G1P) and D-glucose 6-phosphate (G6P), forming beta-D-glucose 1,6-(bis)phosphate (beta-G16P) as an intermediate. The beta-phosphoglucomutase (Beta-PGM) acts on the beta-C1 anomer of G1P. Glucose or lactose are used in preference to maltose, which is only utilized after glucose or lactose has been exhausted. It plays a key role in the regulation of the flow of carbohydrate intermediates in glycolysis and the formation of the sugar nucleotide UDP-glucose. The catalysis proceeds via a phosphoenzyme formed by reaction of an active-site nucleophile with the cofactor glucose 1,6-diphosphate (G1,6-diP). The phosphorylated mutase binds either G1P or G6P and transfers the phosphoryl group to the C6OH or C1OH, respectively.
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基因ID
- 1114041
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UniProt
- P71447
抗原
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