Peroxidase 抗体
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北京 101111
Quick Overview for Peroxidase 抗体 (ABIN105506)
抗原
See all Peroxidase 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-2138-0100
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Supplier
- Rockland
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交叉反应 （详细）
- Cross reactivity against Peroxidase from other tissues and species may occur but have not been specifically determined.
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纯度
- Anti-Peroxidase (Horseradish) (Guinea Pig) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Guinea Pig Serum as well as purified and partially purified Peroxidase [Horseradish].
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内毒素水平
- Low Endotoxin : No
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免疫原
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Peroxidase [Horseradish]
Immunogen Type: Native Protein -
亚型
- IgG
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anti-Peroxidase antibody
适用: Horseradish WB, ELISA, DB, IP 宿主: 山羊 Polyclonal unconjugated
anti-Peroxidase antibody适用: Horseradish WB, ELISA, IHC 宿主: 小鼠 Polyclonal unconjugated
anti-Peroxidase antibody适用: Horseradish WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Anti-Peroxidase (Horseradish) (Guinea Pig) Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity.
ELISA Dilution: 1:20.000 - 1:100.000
Immunohistochemistry Dilution: 1:1.000 - 1:5.000
Western Blot Dilution: 1:2.000 - 1:10.000 -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
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注意事项
- Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
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有效期
- Expiration date is one (1) year from date of opening.
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- Peroxidase
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背景
- Horse Radish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs). Anti-Peroxidase (Horseradish) (Guinea Pig) Antibody is ideal for investigators in Microbiology, Immunology, and Cell Biology research. Synonyms: HRP, Horseradish peroxidase, Guinea pig-anti-peroxidase antibody
抗原
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